Abstract
ELABORATION in vitro of the protective antigen of Bacillus anthracis in serum and plasma was first demonstrated by Gladstone1. Elaboration of the antigen in chemically defined, non-protein media was reported by Wright et al. 2,3Z. The non-protein media facilitated practical production of the antigen, and permitted a basic study of its accumulation and properties. Antigen in the culture filtrate was precipitable with alum; the precipitated product was effective in immunization of animals and was well tolerated in man4–6. Elaboration of protective antigen in chemically defined media was confirmed by Belton and Strange7, who also devised a casein hydrolysate medium suitable for production of antigen.
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References
Gladstone, G. P., Brit. J. Exp. Path., 27, 394 (1946).
Wright, G. G., Hedberg, M. A., and Slein, J. B., J. Immunol., 72, 263 (1954).
Puziss, M., and Wright, G. G., J. Bact., 68, 474 (1954).
Wright, G. G., Green, T. W., and Kanode, R. G., J. Immunol., 73, 387 (1954).
Schlingman, A. S., Devlin, H. B., Wright, G. G., Maine, R. J., and Manning, R. C., Amer. J. Vet. Res., 17, 256 (1956).
Auerbach, S., and Wright, G. G., J. Immunol., 75, 129 (1955).
Belton, F. C., and Strange, R. E., Brit. J. Exp. Path., 35, 144 (1954).
Puziss, M., and Rittenberg, S. C., J. Bact. (in the press).
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WRIGHT, G., PUZISS, M. Elaboration of Protective Antigen of Bacillus anthracis under Anaerobic Conditions. Nature 179, 916–917 (1957). https://doi.org/10.1038/179916a0
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DOI: https://doi.org/10.1038/179916a0
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