An Ion Exchange Method of determining Carnosine, Anserine and their Precursors in Animal Tissue

Abstract

ALTHOUGH elegant procedures have been described for the separation of the neutral and acidic amino-acids by elution from sulphonated polystyrene resins, the quantitative separation of the basic amino-acids has proved more difficult^1,2. For example, when the pH of the eluting buffer is more than 7 their recoveries rarely exceed 80 per cent. It is for this reason that buffers of pH less than 7 and relatively high sodium ion concentration have previously been used in their separation, although such conditions greatly reduce the sensitivity of the method.