Abstract
THE inherent power of the equilibrium method1 for study of molecular weight, polydispersion, and activity coefficients in the ultracentrifuge has been limited by the length of time required to reach equilibrium. We have found that this time can be appreciably reduced by using the synthetic boundary cell2 and by selecting the starting conditions—speed of the ultracentrifuge, the relative heights and the relative concentrations of the two layers—in accordance with a mathematical analysis of the approach to equilibrium.
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PASTERNAK, R., NAZARIAN, G. & VINOGRAD, J. A Fast Method for reaching Equilibrium in the Ultracentrifuge. Nature 179, 92–94 (1957). https://doi.org/10.1038/179092b0
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DOI: https://doi.org/10.1038/179092b0
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