Availability to Dinitrofluorobenzene of some Amino Groups in Human Dentin and Ox Hide Collagen


HUMAN dentin (containing 80 per cent by weight of mineral material), ox hide collagen, and samples of dentin which were partially demineralized at 4° C. by soaking in. either 1 N hydrochloric acid or 1 N trichloracetic acid, or 15 per cent versene (pH 7.3), were assayed for α- and ɛ-amino groups using Sanger's dinitrofluorobenzene method1.

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