Abstract
IN the course of our investigations on the hæmoglobins in the blood of various animal species by the agar electrophoresis technique1,2, it was found that the blood of buffalo contained two distinct hæmoglobins. Blood hæmolysates were prepared by the method of Drabkin3 without using aluminium chloride. The hæmolysate was applied on the surface of the agar gel (0.5 per cent) containing 0.05 ionic strength barbital buffer of pH. 8.6. Electrophoresis was carried out according to the procedure described before4. After electrophoresis (200 V., 6m.amp., 6 hr.) the hæoglobin bands could be observed visually. Under ultra-violet light using Wood's filter the bands could be seen more clearly. The hæmoglobin bands could be stained with amido black 10B after drying the agar plate and the concentrations of the components could be determined densitometrically. The region occupied by the hæmoglobins shows positive reaction with benzidine reagent. Rat blood hæmolysates showed the presence of two minor components of proteins in addition to the major component of hæmoglobin.
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References
Giri, K. V., Naturwiss., 43, 36 (1956).
Giri, K. V., Naturwiss., [43, 232 (1956)]; J. Ind. Inst. Sci., [38, 190 (1956)].
Drabkin, D. L., J. Biol. Chem., 164, 703 (1946).
Giri, K. V., and Pillai, N. C., Curr. Sci., 25, 188 (1956).
Haurowitz, F., Hardin, R. L., and Dicks, M., J. Phys. Chem., 58, 105 (1954).
Harris, H., and Warren, F. L., Biochem. J., 60, xxix (1955).
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GIRI, K., PILLAI, N. Multiple Hæmoglobins in the Blood of Animals. Nature 178, 1057 (1956). https://doi.org/10.1038/1781057a0
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DOI: https://doi.org/10.1038/1781057a0
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