Nucleic Acid Content and Distribution of Chromosome Number in Original and Sublines of Two Mouse Ascites Tumours

Abstract

KOPROWSKI¹ has described the adaptation of the 6C3HED lymphosarcoma², an ascites tumour lethal to C3H mice and to F ₁ hybrids between the original strain and A or DBA mice, to a strain of Swiss mice. Two sublines, designated C3H+ and F ₁, were obtained by injecting embryos of the ICR (Institute for Cancer Research, Philadelphia, Pa.) Swiss mice in utero with blood of either C3H mice or (C3H × DBA) F ₁ hybrids, followed six to eight weeks after birth by the intraperitoneal implantation with cells of the 6C3HED ascites tumour. A third subline, No. 123, was established by injecting ICR mice in the embryonic stage directly with tumour cells followed by the intraperitoneal implantation into adult ICR mice of suspensions of the palpable tumours which developed in the subcutaneous tissue of the neck of mice that had been inoculated in utero.