Abstract
ONE of the essentials of a fluid preparation for nematodes is that coverslip supports must be included in the drop of mounting medium to avoid distortion of the specimens. For this purpose Goodey1 recommends glass fibres arranged radially in the drop; but as the fibres may be of varied and unknown diameter, it is difficult to judge the correct size of drop. If the drop is too large, the lactophenol flows on to the upper surface of the coverslip, and the ringing medium (‘Zut’ or lactophenol gum) will not seal the mount. If the drop is too small, air spaces are left near the edge of the coverslip and the ringing medium is drawn into them by capillarity.
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References
Goodey, T., Technical Bulletin No.2, Min. Agric. and Fish. (H.M.S.O., 1951).
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CAPSTICK, C. Lactophenol Preparations for Soil Nematodes. Nature 177, 896–897 (1956). https://doi.org/10.1038/177896b0
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DOI: https://doi.org/10.1038/177896b0
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