Abstract
IN a previous paper1, evidence was presented for the presence of dominant lethals in X-irradiated haploid yeast cells. Diploid yeast zygotes formed by the conjugation of one irradiated and one un-irradiated haploid cell were found to exhibit signs of radiation damage, including swelling, delay in division and death. The damage responsible for inactivation of these half-irradiated zygotes was defined operationally as dominant lethal damage. The present communication describes a continuation of these experiments in which diploid cells homozygous for mating type were employed along with the two haploid cultures previously used. These diploid cells will conjugate with haploid or diploid cells of opposite mating type when placed in direct contact, thus permitting a similar determination of the frequency of dominant lethals in diploid cells. This was of interest because previous studies of the survival of X-irradiated yeast cells of higher ploidy2 suggested that the frequency of this type of damage might increase with increasing ploidy.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Mortimer, R. K., Radiation Research, 2, 361 (1955).
Mortimer, R. K., U.S. Atomic Energy Commission Document UCRL 1922, pp. 66 (1952).
Ogur, M., Minckler, S., Lindegren, G., and Lindegren, C. G., Arch. Biochem. Biophys., 40, 175 (1952).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
OWEN, M., MORTIMER, R. Dominant Lethality induced by X-Rays in Haploid and Diploid Saccharomyces cerevisiae . Nature 177, 625–626 (1956). https://doi.org/10.1038/177625b0
Issue Date:
DOI: https://doi.org/10.1038/177625b0
This article is cited by
-
The influence of cell ploidy on the thermotolerance of Saccharomyces cerevisiae
Current Genetics (1987)
-
The relevance of the nuclear division cycle to radiosensitivity in yeast
Molecular and General Genetics MGG (1978)
-
Copulation of Ultra-violet-irradiated and Unirradiated Saccharomyces Cells of Different Ploidy
Nature (1966)
-
The problem of evaluating the biological significance of sigmoidal X-ray inactivation curves in yeasts
Il Nuovo Cimento (1960)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.