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Effects of Anaphylactic Shock and Compound 48/80 on the Mast Cells of the Guinea Pig Lung

Abstract

SEVERAL experiments show a correlation between the number of mast cells in a tissue and its content of histamine1, and it is possible to obtain histamine from isolated mast granules3. Furthermore, alterations of mast cells have been described under conditions in which liberation of histamine occurs in the dog, rat and mouse3. We have found that similar changes can be produced in guinea pig lung mast cells by means of anaphylactic shock, but not by the action of compound 48/80. Male guinea pigs weighing 300–450 gm. were used throughout. Groups of five animals each were studied. Group I: animals sensitized by injecting 100 mgm. of commercial egg albumin intraperitoneally and 100 mgm. subcutaneously; anaphylactic shock was induced three weeks later by intracardiac injection of 100 mgm. of the antigen in 1 ml. of 0.85 per cent sodium chloride. Group II: sensitized animals killed after shock was induced by intracardiac injection of 200γ of histamine. This group was chosen in order to obtain lungs in similar conditions (emphysematous) to those of group I. Group III: animals injected through the intracardiac route with 0.5 mgm. of compound 48/80. The animals from these three groups were killed by bleeding from the jugular vein when breathing had practically stopped. Group IV : animals killed by a blow on the head and bled from the jugular vein.

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MOTA, I., VUGMAN, I. Effects of Anaphylactic Shock and Compound 48/80 on the Mast Cells of the Guinea Pig Lung. Nature 177, 427–429 (1956). https://doi.org/10.1038/177427a0

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