Cytoplasmic Structures in Yeasts

Abstract

THE above comment on our papers starts with the assertion that “Yotsuyanagi has reported long, curved, cylindrical, occasionally branched, thread-like mitochondria in yeast cells by staining with tetrazolium”. This is incorrect; the thread-like mitochondria referred to were stained with janus green and with Altmann's fuchsin. The observation of Williams, Lindegren and Yuasa that “deposits of formazan, especially the threadlike structures, are dye crystals not resembling any normal structure present in the yeast cell” is therefore irrelevant.