Effect of Growth Hormone on the Large-Particle Content of Rat Liver

Abstract

IN the course of an investigation1 of rat liver, with respect to hormonal effects on the yield and composition of fractions isolated by differential centrifugation in 0.25 M sucrose medium2, determinations have been made of the particle number in freshly isolated ‘large-particle’ (‘mitochondrial’) fractions. These fractions were obtained from liver homogenates, prepared with a Potter-type homogenizer and centrifuged at a low speed to remove ‘debris’ containing the nuclei, by farther centrifugation for 20 min. in an ‘Angle 13’ (Measuring and Scientific Equipment, Ltd.) centrifuge at 12,000 g (maximum value, at extreme tip of tube). The particles were suspended in 1.8 M sucrose solution and counted under dark-ground illumination as described by Allard et al. 3. Under these conditions (in 1.8 M sucrose) the particle size-range is decreased (compressed); all visible particles up to and including the mitochondrial size-range were counted.

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References

  1. 1

    Reid, E., (a) Biochem. J., 58, xxiii (1954); (b) Proc. Symp. on “The Hypophyseal Growth Hormone” (Henry Ford Hospital, Detroit) (to be published); (c) Nature, [175, 461 (1955 )].

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  2. 2

    Schneider, W. C., J. Biol. Chem., 176, 259 (1948).

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  3. 3

    Allard, C., Mathieu, R., de Lamirande, G., and Cantero, A., Cancer Research, 12, 407 (1952).

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GREENBAUM, A., SLATER, T. & REID, E. Effect of Growth Hormone on the Large-Particle Content of Rat Liver. Nature 175, 506–507 (1955). https://doi.org/10.1038/175506b0

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