Abstract
BULL semen for artificial insemination is usually stored at 4° C. in media composed of equal volumes of either phosphate buffer (pH 7.4) or citrate buffer (pH 6.8) and fresh egg-yolk1. Under these conditions the motility and fertilizing capacity of spermatozoa decrease rapidly with time, and in practice samples are not used for insemination for more than three days after collection. Semen can be successfully stored for much longer periods at − 79° C.2, but this technique has disadvantages for everyday use.
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ROY, A., BISHOP, M. Effect of Glycine on the Survival of Bull Spermatozoa in vitro . Nature 174, 746–747 (1954). https://doi.org/10.1038/174746a0
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DOI: https://doi.org/10.1038/174746a0
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