Abstract
IT is generally accepted that only direct microscopy is able to give a reliable estimate of the total numbers of micro-organisms present in soil. Suitable direct techniques have been developed for counting the bacteria, fungi1 and algae2. No adequate technique is available for the protozoa. A recently developed culture technique3 is a considerable improvement, but tends to be selective and is time-consuming in operation.
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Jones, P. C. O., and Mollison, J. E., J. Gen. Microbiol., 2, 54 (1948).
Tchan, Y. T., Nature, 170, 328 (1952).
Singh, B. N., Nature, 157, 302 (1946).
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TCHAN, Y., BUNT, J. Direct Microscopy for Study and Count of Soil Protozoa. Nature 174, 656 (1954). https://doi.org/10.1038/174656a0
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DOI: https://doi.org/10.1038/174656a0
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