Abstract
WHILE investigating the chromatographic separation of sugars upon filter paper impregnated with sodium borate buffer (0.05 N borax, pH 9.18) it was found that the carbohydrates showed up prominently on spraying with 80 per cent aqueous ethanol containing either 1 mgm./ml. of phenolphthalein or phenol red just made alkaline with sodium hydroxide. Phenolphthalein gave rise to white spots on a purple background and was rather more effective than phenol red, which gave yellow spots on a purple background.
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References
Jeanes, A., Wise, S. S., and Dimler, R. J., Anal. Chem., 23, 415 (1951).
Bradfield, A. E., and Flood, A. E., Nature, 166, 264 (1950).
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HOCKENHULL, D. A New Spraying Agent for Paper Chromatography of Carbohydrates and Polyols. Nature 171, 982 (1953). https://doi.org/10.1038/171982a0
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DOI: https://doi.org/10.1038/171982a0
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