Abstract
THE recent communication by Phillips and Pollard1 suggests that results obtained here may be of interest. In the paper chromatography of dilute and very impure sugar solutions, such as non-diabetic urines or certain protein hydrolysates, it is common practice to use beforehand a ‘mixed bed’ ion-exchange resin to remove interfering anions and cations2. For example, ‘Bio-Deminrolit E’ a mixture of a weakly basic resin containing amine groups and a sulphonic acid type resin, is shaken with the solution for 20 min. and, after centrifuging, the clear supernatant contains all the sugars (except amino-sugars and uronic acids) uncontaminated by ionizable substances3. When ‘Bio-Deminrolit E’ was replaced by ‘Bio-Deminrolit’ or ‘Bio-Deminrolit F’, which are mixtures of sulphonic acid type resins with strongly basic resins of the quaternary ammonium hydroxide type, it was found that all fructose, glucose, galactose, mannose and fucose were removed from the dilute solutions employed.
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References
Phillips, J. D., and Pollard, A., Nature, 171, 42 (1953).
Partridge, S. M., Biochem. J., 42, 238 (1948).
Woolf, L., Great Ormond Street J., 1, 61 (1951).
Piez, K. A., Tooper, E. B., and Fosdick, L. S., J. Biol. Chem., 194, 669 (1952).
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WOOLF, L. Use of Ion-Exchange Resins in Paper Chromatography of Sugars. Nature 171, 841 (1953). https://doi.org/10.1038/171841a0
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DOI: https://doi.org/10.1038/171841a0
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