Abstract
EARLIER investigations of the action of insulin on cold-blooded vertebrates have shown that, although large doses usually lead to the appearance of typical convulsions, there is often a long latent period (1–2 days at room temperature) during which there are no visible symptoms1–3. In experiments performed in March and May 1952 the action of insulin on the blood-sugar level of the frog was followed, at intervals, from the time of injection to the development of convulsions. The frogs were kept at room temperature (17°–20° C.) and were injected with four units of insulin (Wellcome 40) in the dorsal lymph sac. The sugar content of the blood was determined by a modification of the method described by King4 for obtaining “true sugar” values, which are 15–20 mgm./100 ml. lower than those previously recorded for the frog5.
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References
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Gray, I. E., and Hall, F. G., Biol. Bull., 58, 217 (1930).
King, E. J., “Micro-analysis in Medical Biochemistry” (J. and A. Churchill, London, 1946).
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SMITH, C. Action of Insulin on the Frog (Rana temporaria). Nature 171, 311–312 (1953). https://doi.org/10.1038/171311b0
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DOI: https://doi.org/10.1038/171311b0
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