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Cord Blood Stem Cells

Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation

Bone Marrow Transplantation volume 40, pages 837–842 (2007)Cite this article

Abstract

Optimum storage conditions of cord blood-derived hematopoietic progenitor cells before isolation remain unknown. We therefore evaluated CD34+ cells isolated from cord blood units (n=57) within 1 h after collection and following storage for 24, 48 and 72 h at either room temperature (RT) or 4 °C. Isolated CD34+ cells were analyzed for their cell count, immunophenotype, apoptosis rate, clonogenicity and transmigration capacity in response to stroma-derived factor 1α using direct-paired comparisons (n=27). CD34+, CD133+ and CD45+ positivity after isolation remained the same under all conditions. After 24 h, CD34+ cell counts and numbers of CFU-GM colonies dropped regardless of the storage temperature. After 48 h, the number of CD34+ cells increased compared to 24 h, if the cord blood had been stored at RT resulting in almost three times more CD34+ cells than at 4 °C. These cells had a lower early apoptosis rate and formed four times more BFU-E than those stored at 4 °C with equivalent plating efficiencies. CD34+ cells kept at RT for 48 h had the highest transmigration capacities, which paralleled an increased CXCR-4 expression. Cord blood should be stored at RT before CD34+ isolation and a storage time for 48 h should be preferred to 24 h.

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Acknowledgements

This study was supported by the Federal Ministry of Education and Research (Grant 0311591). We are indebted to the nurses and doctors, especially Jens Stupin and Gabriele Gossing, of the obstetric department of the Charité for providing the cord blood units. We also acknowledge Imad Zalloum for excellent technical support.

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Authors and Affiliations

  1. Institute for Transfusion Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany

    A Moldenhauer, J Wolf, G Genter, H Kiesewetter & A Salama

  2. Institute for Molecular Biophysics, Humboldt University, Berlin, Germany

    G Habermann

Authors
  1. A Moldenhauer
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  2. J Wolf
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  3. G Habermann
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  4. G Genter
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  5. H Kiesewetter
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  6. A Salama
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Correspondence to A Moldenhauer.

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Supplementary Information accompanies the paper on Bone Marrow Transplantation website (http://www.nature.com/bmt)

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Moldenhauer, A., Wolf, J., Habermann, G. et al. Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation. Bone Marrow Transplant 40, 837–842 (2007). https://doi.org/10.1038/sj.bmt.1705831

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