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CD34+ Cell Isolation

Allogeneic peripheral blood stem cell transplantation following CD34+ enrichment by density gradient separation

Bone Marrow Transplantation volume 25, pages 1223–1228 (2000)Cite this article

Abstract

GVHD is a significant cause of morbidity and mortality following allogeneic peripheral blood stem cell transplantation (AlloPBSC). CD34+ cell selection could reduce GVHD by negative selection of T cells. In an attempt to reduce the T cell content of alloPBSC we carried out a trial in which 11 patients with hematologic malignancies received alloPBSC from HLA-matched siblings following density gradient separation using an isotonic colloidal silica solution (BDS 60; Dendreon Corporation). Cyclosporine and methylprednisone were used for GVHD prophylaxis. The mean yield of CD34+ cells was 69 ± 15.6% with a purity of 2.9 ± 1.7%. The mean number of CD3+ cells infused was 1.0 ± 1.2 × 107/kg, representing a 1.3 log depletion. A high risk of acute GVHD was observed: grade II–IV in 7/11 (64%) and grade III–IV GVHD in 5/11 (45%) patients. Nine of the 11 (82%) patients died with a median survival of 68 days. Cytokine expression in PBSC was compared pre and post processing. Interferon-gamma was detected only following density gradient separation while IL-8 expression increased 3- to 6-fold post processing. Therefore, processing with this device may augment production of pro-inflammatory cytokines. Bone Marrow Transplantation (2000) 25, 1223–1228.

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Authors and Affiliations

  1. Divisions of Bone Marrow Transplantation and Stem Cell Biology, Washington University School of Medicine, St Louis, MO, USA

    R Vij, R Brown, JS Haug, D Adkins, LT Goodnough, H Khoury & J DiPersio

  2. Divisions of Pediatric Hematology Oncology, Washington University School of Medicine, St Louis, MO, USA

    S Shenoy & D Kaesberg

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  1. R Vij
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  2. R Brown
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  3. S Shenoy
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  4. JS Haug
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  5. D Kaesberg
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  6. D Adkins
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  7. LT Goodnough
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  8. H Khoury
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  9. J DiPersio
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Vij, R., Brown, R., Shenoy, S. et al. Allogeneic peripheral blood stem cell transplantation following CD34+ enrichment by density gradient separation. Bone Marrow Transplant 25, 1223–1228 (2000). https://doi.org/10.1038/sj.bmt.1702427

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  • DOI: https://doi.org/10.1038/sj.bmt.1702427

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