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Viral Infections

Human herpesvirus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients

Bone Marrow Transplantation volume 25, pages 153–160 (2000)Cite this article

Abstract

Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus (KSHV), has recently been identified within the bone marrow dendritic cells of multiple myeloma (MM) patients. This virus contains homologues to human cytokines such as IL-6 that could potentially stimulate myeloma cell growth and contribute to disease pathogenesis. Since mobilization chemotherapy may increase circulating dendritic cell numbers, we searched for HHV-8 in peripheral blood mononuclear cells (PBMCs) before and after mobilization chemotherapy given to MM patients. Furthermore, we determined if autograft purging using the CEPRATE SC device would reduce the percentage of HHV-8 infected stem cell products. Only two of the 39 PBMC samples collected prior to mobilization chemotherapy contained PCR detectable virus, yet nine of 37 PBMCs collected on the first day of leukapheresis had detectable HHV-8 (P = 0.016). HHV-8 was more frequently identified in autograft products before vs after Ceprate SC selection (40% vs 15%, P = 0.016). Although the role HHV-8 plays in myeloma pathogenesis remains unclear, these results imply that mobilization chemotherapy increases the numbers of circulating HHV-8-infected dendritic cells within the peripheral blood. In addition, CD34 selection of autograft products in MM patients may reduce the reintroduction of virally infected cells following high-dose chemotherapy. Bone Marrow Transplantation (2000) 25, 153–160.

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Authors and Affiliations

  1. West LA VAMC/University of California, Los Angeles, CA, USA

    RA Vescio, CH Wu, L Zheng, D Sheen, H Ma, J Liu, GJ Schiller & JR Berenson

  2. The Toronto Hospital, Toronto, Canada

    AK Stewart

  3. University of South Florida, Miami, FL, USA

    O Ballester

  4. Johns Hopkins University, Baltimore, MD, USA

    SJ Noga

  5. UC San Francisco, San Francisco, CA, USA

    H Rugo

  6. University of Texas at San Antonio, TX, USA

    C Freytes

  7. University of Pennsylvania, Philadelphia, PA, USA

    E Stadtmauer

  8. Southern California Kaiser Permanente Group, Los Angeles, CA, USA

    F Sahebi

  9. University of Nebraska, Omaha, NE, USA

    S Tarantolo

  10. Loyola University, Chicago, IL, USA

    P Stiff

  11. CellPro Inc., Bothell, WA, USA

    M White & C Jacobs

  12. Washington University School of Medicine, St Louis, MO, USA

    J DiPersio

  13. Dana Farber Cancer Institute, Boston, MA, USA

    KC Anderson

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Vescio, R., Wu, C., Zheng, L. et al. Human herpesvirus 8 (KSHV) contamination of peripheral blood and autograft products from multiple myeloma patients. Bone Marrow Transplant 25, 153–160 (2000). https://doi.org/10.1038/sj.bmt.1702113

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  • DOI: https://doi.org/10.1038/sj.bmt.1702113

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