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Immunobiology

A high resolution HLA class I and class II matching method for bone marrow donor selection

Bone Marrow Transplantation volume 22, pages 527–534 (1998)Cite this article

Abstract

HLA matching in bone marrow transplantation has an important role in determining successful outcome. However HLA typing of both potential related and unrelated donors can be both time-consuming and laborious, and does not always resolve accurately the true level of histocompatibility. We have utilised a method, reference strand mediated conformation analysis (RSCA), which is technically simple and allows high resolution matching for all HLA loci, for the typing of 48 patients and their potential 120 donors. The results indicate that RSCA can detect many mismatches that are not routinely identified by conventional HLA typing methods. In addition, RSCA can be applied for the simultaneous analysis of multiple potential BM donor samples in order to quickly identify the best match for each patient.

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Authors and Affiliations

  1. Anthony Nolan Research Institute, The Royal Free Hospital School of Medicine, London, UK

    JR Argüello, A-M Little, E Bohan, D Gallardo, J O’Shea, IA Dodi, JM Goldman & JA Madrigal

  2. Department of Haematology, The Royal Free Hospital School of Medicine, London, UK

    A-M Little & JA Madrigal

  3. The Imperial College School of Medicine, London, UK

    JM Goldman & JA Madrigal

Authors
  1. JR Argüello
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  2. A-M Little
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  3. E Bohan
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  4. D Gallardo
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  5. J O’Shea
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  6. IA Dodi
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  7. JM Goldman
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  8. JA Madrigal
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Argüello, J., Little, AM., Bohan, E. et al. A high resolution HLA class I and class II matching method for bone marrow donor selection. Bone Marrow Transplant 22, 527–534 (1998). https://doi.org/10.1038/sj.bmt.1701383

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  • DOI: https://doi.org/10.1038/sj.bmt.1701383

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