Abstract
A METHOD of assay of glucose-6-phosphate de-hydrogenase in liver has been devised employing spectrophotometric measurement of the rate of reduction of triphosphopyridine nucleotide in suitably prepared liver homogenate supernatants. This is preferable to the determination of dehydrogenase activity in extracts of acetone-dried tissues1, or to the use of unphysiological carriers such as brilliant cresyl blue, 2-6-dichlorophenolindophenol or phenazine methosulphate2.
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References
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GLOCK, G., McLEAN, P. Glucose-6-Phosphate Dehydrogenase Activity of Rat Liver. Nature 170, 119–120 (1952). https://doi.org/10.1038/170119a0
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DOI: https://doi.org/10.1038/170119a0
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