Ionophoretic Separation of Porphyrin Pigments

Abstract

IN the classical Tiselius procedure for electrophoresis of proteins, convection was prevented by the density gradient of the substances under investigation; but complete separation could not thereby be obtained, and the analysis was conducted by the observation of fronts. Later the medium was stabilized by silica or agar gel for the separation of amino-acids¹ and proteins². Silica or agar gel will hold an excess of electrolyte and prevent convection currents; but it will allow substances to pass through independently and to separate into discrete bands. In addition, its use obviates contact of the moving substances with the electrodes.