Abstract
PAPER chromatography of the p-nitrobenzene-azodimethoxyaniline (‘Fast Black Salt K’) derivatives of two commercial samples of œstrone, according to the method described by Heftmann1, revealed the presence of small traces of an impurity which appeared as a blue spot with lower RF value than the pink œstrone dye. This substance could be removed from the œstrone by means of partition chromatography on a ‘Celite’–sodium hydroxide column, following the method of Swyer and Braunsberg2. Paper chromatograms, run before and after purification by our technique, showed that the impurity was completely removed by this means (see photograph).
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References
Heftmann, E., Science, 111, 571 (1950); J. Amer. Chem. Soc., 73, 851 (1951).
Swyer, G. I. M., and Braunsberg, H., J. Endocrin., 7, lx (1951).
Mentioned by Carol, J., J. Amer. Pharm. Assoc., Sci. Ed., 39, 425 (1951).
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BRAUNSBERG, H. Purification of Commercial Samples of Œstrone. Nature 169, 967–968 (1952). https://doi.org/10.1038/169967b0
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DOI: https://doi.org/10.1038/169967b0
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