Spectrophotometric Determination of Hæmoglobin in Blood


A VARIETY of methods has been used for the determination of hæmoglobin in blood. They are based on (i) oxygen absorption1, (ii) the iron content2, or (iii) the transformation of the hæmoglobin to carboxyhæmoglobin3, cyanhæmatin or acid hæmatin, which are then estimated colorimetrically4 or spectroscopically5,6.

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    Van Slyke, O. D., and Neill, J. M., J. Biol. Chem., 61, 523 (1924).

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    Wong, J. Biol. Chem., 77, 409 (1928).

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    King, E. J., and Geiser, M., Biochem. J., 46, Proc. xxv (1950).

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    Sahli, H., “Diagnostic Methods”, Translation of edit. 4 (Philadelphia, 1905).

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    Newcomer, H. S., J. Biol. Chem., 37, 465 (1919).

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    Heilmayer, L., “Spectrophotometry in Medicine” (Adam Hilger, Ltd., 1943).

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KHALIFA, A., SALAH, M. Spectrophotometric Determination of Hæmoglobin in Blood. Nature 168, 915–916 (1951). https://doi.org/10.1038/168915a0

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