Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Spectrophotometric Determination of Hæmoglobin in Blood

Abstract

A VARIETY of methods has been used for the determination of hæmoglobin in blood. They are based on (i) oxygen absorption1, (ii) the iron content2, or (iii) the transformation of the hæmoglobin to carboxyhæmoglobin3, cyanhæmatin or acid hæmatin, which are then estimated colorimetrically4 or spectroscopically5,6.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Van Slyke, O. D., and Neill, J. M., J. Biol. Chem., 61, 523 (1924).

    CAS  Google Scholar 

  2. Wong, J. Biol. Chem., 77, 409 (1928).

    CAS  Google Scholar 

  3. King, E. J., and Geiser, M., Biochem. J., 46, Proc. xxv (1950).

  4. Sahli, H., “Diagnostic Methods”, Translation of edit. 4 (Philadelphia, 1905).

    Google Scholar 

  5. Newcomer, H. S., J. Biol. Chem., 37, 465 (1919).

    CAS  Google Scholar 

  6. Heilmayer, L., “Spectrophotometry in Medicine” (Adam Hilger, Ltd., 1943).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

KHALIFA, A., SALAH, M. Spectrophotometric Determination of Hæmoglobin in Blood. Nature 168, 915–916 (1951). https://doi.org/10.1038/168915a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/168915a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing