Abstract
IN a recent publication1 from this laboratory, a technique for the study of the chromatographic behaviour of enzymes on paper was described. The ease with which enzymes can be readily identified on the paper chromatogram by the agar-plate method enabled us to study the chromatographic behaviour of some of the important enzymes. In the course of preliminary experiments with amylases, phos-phorylases and phosphatases from various sources, we found that some enzymes did not move at all while others moved considerable distances on the paper. This observation prompted us to attempt the separation of enzymes by paper chromatography.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Giri, K. V., and Prasad, A. L. N., Nature, 167, 859 (1951).
Mitchell, H. K., Gordon, M., and Haskins, F. A., J. Biol. Chem., 180, 1071 (1949).
Wallenfels, K. W., and Pechman, E. V., Ang. Chem., 63, 44 (1951).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GIRI, K., PRASAD, A. Separation of Enzymes by Paper Chromatography. Nature 168, 786–787 (1951). https://doi.org/10.1038/168786b0
Issue Date:
DOI: https://doi.org/10.1038/168786b0
This article is cited by
-
Formation of amino acids by the photochemical method using various carbon-containing substances in presence of nickel as catalyst
Bulletin of the Academy of Sciences of the USSR Division of Chemical Science (1962)
-
Partition chromatography and its use in the plant sciences
The Botanical Review (1959)
-
Formation of amino acids in water containing dissolved carbon dioxide and colloidal molybdenum oxide under the influence of artificial light
Bulletin of the Academy of Sciences of the USSR Division of Chemical Science (1958)
-
Photosynthesis of Amino-Acids from Paraformaldehyde and Potassium Nitrate
Nature (1954)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.