Abstract
CONSDEN et al.1 observed that in the case of phenol saturated with water as the developing solvent, RF values of amino-acids increase with increase of temperature, since the water-content of phenol varies directly with temperature within the range studied. The reverse is the case with collidine, the water-content of which decreases with increase of temperature. The water-content of solvents which are completely miscible with water can be suitably adjusted, the phase-composition remaining unaffected by temperature. If the change in phase-composition of the developing solvent is the only cause of alterations of RF values with temperature, they should be independent of temperature in case of miscible solvents. That this is not so will be clear from the following.
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References
Consden, R., Gordon, A. H., and Martin, A. J. P., Biochem. J., 38, 224 (1944).
Williams, R. J., and Kirby, H. M., Science, 107, 481 (1948).
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BURMA, D. Effect of Temperature on the RF Values of the Amino-acids during Paper Chromatography with Solvents completely Miscible with Water. Nature 168, 565–566 (1951). https://doi.org/10.1038/168565b0
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DOI: https://doi.org/10.1038/168565b0
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