Abstract
THE only dinitrophenyl amino-acids revealed by paper chromatography1 and the use of silica columns2 during an attempt to identify and estimate the free amino groups of lysozyme by the method of Sanger2 were σ-dinitrophenyl-lysine and bis-dinitrophenyl-lysine. The recovery of the former from hydrolysed dinitrophenyl-lysozyme, using silica columns, suggested that lysozyme contains five non-terminal lysine residues; but the amount of bis-dinitrophenyl-lysine recovered was only one-third of that to be expected from one N-terminal amino-group. Similar results were obtained when lysozyme was first treated with urea3, or when the conditions of reaction with 1 : 2 : 4-fluorodinitrobenzene were varied.
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References
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Lewis, J. C., Snell, N. S., Hirschmann, D. J., and Fraenkel-Conrat, H., J. Biol. Chem., 186, 23 (1950).
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THOMPSON, A. Destruction of Dinitrophenyl Amino-Acids by Tryptophane. Nature 168, 390–391 (1951). https://doi.org/10.1038/168390a0
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DOI: https://doi.org/10.1038/168390a0
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