Abstract
THE recent report1 by Hamer on the amino-acid composition of calf thymus histone prompts us to communicate some corresponding observations on a histone prepared from rat liver deoxypentosenucleoprotein. Nuclei were isolated from briefly homogenized suspensions of perfused rat liver (Sprague–Dawley–Holtzmann strain) and washed four times. A citric acid technique at pH 6.1 was employed in the preparation2. The nuclei were subjected to differential extraction with sodium chloride, 1.0 M salt being used for the extraction of the deoxypentosenucleoprotein and dilution to 0.14 M salt for its precipitation. Unlike the preparations described in the literature, these are non-fibrous on precipitation, readily soluble in water, non-viscous in either water or molar sodium chloride solution, and of low molecular weight. The deoxypentosenucleoprotein was dialysed against water and the solution so obtained was lyophilized.
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References
Hamer, D., Nature, 167, 40 (1951).
Dounce, A. L., J. Biol. Chem., 147, 685 (1943).
Stein, W. H., and Moore, S., J. Biol. Chem., 176, 337 (1948).
Moore, S., and Stein, W. H., J. Biol. Chem., 176, 367 (1948).
Moore, S., and Stein, W. H., J. Biol. Chem., 178, 53 (1949).
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BRUNISH, R., FAIRLEY, D. & LUCK, J. Composition of Histone prepared from Rat Liver Deoxypentosenucleoprotein. Nature 168, 82–83 (1951). https://doi.org/10.1038/168082a0
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DOI: https://doi.org/10.1038/168082a0
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