Chromatography of Proteins

Abstract

WE have been concerned with the chromatographic separation of proteins and have developed a simple method of location of proteins on a paper chromatogram or a cellulose column. We have utilized the fact that acid dyes have an affinity for protein fibres but not for cellulose. The developed chromatogram is treated with an acid solution of a suitable dyestuff, preferably warmed for a few minutes, then rinsed in water, when the proteins appear as coloured spots or streaks on a white background. Amino-acids and peptides are not stained.

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JONES, J., MICHAEL, S. Chromatography of Proteins. Nature 165, 685 (1950) doi:10.1038/165685a0

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