Abstract
IN a search for an agent for revealing the position of choline and other N-methylated alkanolamines separated by chromatography on papers, iodine was found satisfactory. It can be either directly sublimed on to the dried paper or sprayed in alcoholic solution, methods having their special advantages. during the sublimation or spraying, spots of a brown to yellow colour develop, and they grow more distinct when any excess iodine or solvent is fanned away. Inspection in ultra-violet light sometimes makes the border-lines of the spots more easily discernible. As fading rapidly sets in, the spots are best judged immediately and outlined with pencil. After some heating or hanging at room temperature, the colour of the spots caused by most substances disappears completely. New iodine treatment then again reveals them.
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Brante, G. Iodine as a Means of Development in Paper Chromatography. Nature 163, 651–652 (1949). https://doi.org/10.1038/163651b0
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DOI: https://doi.org/10.1038/163651b0