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Aneurin-Pyrophosphate Content of Red and White Blood Corpuscles in the Rat and in Man

Nature volume 162, pages 220221 (07 August 1948) | Download Citation

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Abstract

WE have determined accurately, the aneurinpyrophosphate content of red and white blood corpuscles in the rat and in man. Rats in a well-nourished condition and with slight aneurin deficiency were examined, and also human subjects, both healthy and suffering from various diseases. Determinations were carried out in two blood fractions, obtained by centrifuging, one containing a large number of red cells and very few white cells, the second containing practically all the leucocytes of the blood sample and relatively few erythrocytes. Centrifuging must be carried out at a low rate, as at high rates the white cells agglutinate and cannot be resuspended for counting. Cell counting1 must be performed with great care, as it is subject to much larger errors than the aneurin-pyrophosphate determination. The latter was carried out according to Westenbrink et al.2.

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References

  1. 1.

    , and , Ned. Tijdschr. Geneesk., 91, 3292 (1947).

  2. 2.

    , , , and , Z. Vitaminforsch., 13, 218 (1943).

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Affiliations

  1. Laboratory of Physiological Chemistry of the University and Central Institute for Nutrition Research, T.N.O., Utrecht. April 6.

    • E. FLORIJN
    •  & G. SMITS

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DOI

https://doi.org/10.1038/162220a0

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