Abstract
Bacteria have usually been prepared for observation in the electron microscope by growth in liquid media, a drop of the suspension being allowed to dry on a collodion-covered specimen grid. Smiles1 has employed an adaptation of the impression technique used in optical microscopy for obtaining material from a surface culture: a glass slide pressed on to the agar removes a film of bacteria, which is allowed to dry partially, and then removed by stripping with a collodion film. Hillier and Baker2 have described a more direct method, in which the collodion film is formed on the agar surface and then floated off in water. Unfortunately, most bacteria, however mounted, prove to be too opaque to an electron beam of the energy usually employed (50–60 kV.) to show appreciable internal structure.
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References
Smiles (demonstrated to the third Electron Microscope Conference National Institute for Medical Research, London, June 1945).
Hillier and Baker, J. Bact., 52, 411 (1946).
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BRIEGER, E., CROWE, G. & COSSLETT, V. Electron Microscopy of Bacteria. Nature 160, 864 (1947). https://doi.org/10.1038/160864a0
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DOI: https://doi.org/10.1038/160864a0
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