Abstract
Background: Vitamin A deficiency (VAD) is endemic in Brazil and health professionals have difficulty in recognizing its subclinical form. In addition, serum retinol concentrations do not always represent vitamin A status in the organism.
Objective: To identify VAD in preschool children by the serum 30-day dose–response test (+S30DR) and to examine its potential as a tool for the assessment of vitamin A status in the community.
Design: A prospective transverse study in which blood samples were obtained from 188 preschool children for the determination of serum retinol concentrations and the children were submitted to ocular inspection and anthropometric measurements. Information about the presence of diarrhea and/or fever during the 15 days preceding the study was also obtained. The children received an oral dose of 200,000 IU vitamin A immediately after the first blood collection. A second blood sample was obtained 30–45 days after supplementation in order to determine the +S30DR.
Results: In all, 74.5% (140/188; 95% confidence interval: 68.3–80.7%) of the children presented +S30DR values indicative of low hepatic reserves. The mean serum retinol concentration was significantly lower before supplementation (0.92 and 1.65 μmol/l, respectively; P<0.0001). No child presented xerophthalmia; 3.7% (7/188) of the children were malnourished. The presence of fever and/or diarrhea during the 15 days preceding the first blood collection did not affect the +S30DR value.
Conclusions: The prevalence of VAD in the study group was elevated. +S30DR proved to be a good indicator of subclinical VAD in children from an underdeveloped country.
Sponsorship: The study was supported by School of Medicine of Ribeirão Preto, University of São Paulo, São Paulo, Brazil.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Akinyinka OO, Usen SOI, Akanni AO, Falade AG & Olumese PE (2000): Risk factors for low serum retinol in Nigerian children. J. Trop. Pediatr. 46, 250–251.
Alvarez JO, Salazar-Lindo E, Kohatsu J, Miaranda P & Stephensen CB (1995): Urinary excretion of retinol in children with acute diarrhea. Am. J. Clin. Nutr. 61, 1273–1276.
Amédée-Manesme O, Anderson D & Olson JA (1984): Relation of the relative dose response to liver concentrations of vitamin A in generally well-nourished surgical patients. Am. J. Clin. Nutr. 39, 898–902.
Amédée-Manesme O, Mourey MS, Hanck A & Therasse J (1987): Vitamin A realtive dose response test: validation by intravenous injection in children with liver disease. Am. J. Clin. Nutr. 46, 286–289.
Arnaud J, Fortis I, Blachier S, Kia D & Favier A (1991): Simultaneous determination of retinol, α-tocopherol and β-carotene in serum by isocratic high-performance liquid chromatography. J. Chromatogr. 572, 103–126.
Ballew C, Bowman BA, Sowell AL & Gillespie C (2001): Serum retinol distributions in residents of the United States: third National Health and Nutrition Examination Survey, 1988–1994. Am. J. Clin. Nutr. 73, 586–593.
Baqui AH, Black RE, Yunus MD, Hoque ARZ, Chowdhury HR & Sack RB (1991): Methodological issues in diarrhoeal diseases epidemiology: definition of diarrhoeal episodes. Int. J. Epidemiol. 20, 1057–1063.
Bloem MW, Wedel M, Egger RJ, Speek AJ, Schrijver J, Saowakontha S & Schreurs WHP (1990): Mild vitamin A deficiency and risk of respiratory tract diseases and diarrhea in preschool and school children in northeastern Thailand. Am. J. Epidemiol. 131, 332–339.
Caballero E, Rivera G & Nelson DP (1996): Encuesta nacional sobre la vitamina A en Panamá. Bol. Ofic. Sanit. Panam. 120, 181–188.
Castejón HV, Ortega P, Díaz ME, Amaya D, Gómez G, Ramos M, Alvarado MV & Urrieta JR (2001): Prevalencia de deficiencia subclínica de vitamina A y desnutrición en niños marginales de Maracaibo – Venezuela. Arch. Latinoam. Nutr. 51, 25–32.
De Pee S & Dary O (2002): Biochemical indicators of vitamin A deficiency: serum retinol and serum retinol binding protein. J. Nutr. 132 (Suppl), S2895–S2901.
Donnen P, Brasseur D, Dramaix M, Vertongen F, Ngoy B, Zihindula M & Hennart P (1996): Vitamin A deficiency and protein-energy malnutrition in a sample of pre-school age children in the Kivu Province in Zaire. Eur. J. Clin. Nutr. 50, 456–461.
Drott P, Meurling S & Gebre-Medhin M (1993): Interactions of vitamins A and E and retinol-binding protein in healthy Swedish children — evidence of thresholds of essentiality and toxicity. Scand. J. Clin. Lab. Invest. 53, 275–280.
Ferraz IS, Daneluzzi JC & Vannucchi H (2000): Vitamin A deficiency in children aged 6 to 24 months in São Paulo state, Brazil. Nutr. Res. 20, 757–768.
Flores H (1993): Frequency distributions of serum vitamin A levels in cross-sectional surveys and in surveys before and after vitamin A supplementation. In: A Brief Guide to Current Methods of Assessing Vitamin A Status, A Report of the International Vitamin A Consultative Group (IVACG), ed. BA Underwood & JA Olson. Washington, DC: The Nutrition Foundation Inc.
Flores H, Azevedo MNA, Campos FACS, Barreto-Lins MC, Cavalcanti AA, Salzano AC, Varela A & Underwood BA (1991): Serum vitamin A distribution curve for chidren aged 2–6 y known to have adequate vitamin A status: a reference population. Am. J. Clin. Nutr. 54, 707–711.
Flores H, Campos F, Araújo CRC & Underwood BA (1984): Assessment of marginal vitamin A deficiency in Brazilian children using the relative dose response procedure. Am. J. Clin. Nutr. 40, 1281–1289.
Friis H, Ndhlovu P, Kaondera K, Sandstrom B, Michaelsen KF, Vennervald BJ & Christensen NO (1996): Serum concentration of micronutrients in relation to schistosomiasia and indicators of infection: a cross-sectional study among rural Zimbabwean schoolchildren. Eur. J. Clin. Nutr. 50, 386–391.
Gilbert C & Foster A (2001): Childhood blindness in the context of VISION 2020 — the right to sight. Bull. World Health Organ. 79, 227–232.
Goodman T, Dalmiya N, De Benoist B & Schultink W (2000): Polio as a platform: using national immunization days to deliver vitamin A supplements. Bull. World Health Organ. 78, 305–314.
Kapil U & Bhavna M (2002): Adverse effects of poor micronutrients status during childhood and adolescence. Nutr. Rev. 60 (Suppl 2), S84–S90.
Kello AB & Gilbert C (2003): Causes of severe visual impairment and blindness in chidren in schools for the blind in the Ethiopia. Br. J. Ophthalmol. 87, 526–530.
Loerch JD, Underwood BA & Lewis KC (1979): Response of plasma levels of vitamin A to a dose of vitamin A as an indicator of hepatic vitamin A reserves in rats. J. Nutr. 109, 778–786.
Miller M, Humphrey J, Johnson E, Marinda, E Brookmeyer R & Katz J (2002): Why do children become vitamin A deficient? J. Nutr. 132 (Suppl), S2867–S2880.
Milton RC, Reddy V & Naidu AN (1987): Mild vitamin A deficiency and childhood morbidity — an Indian experience. Am. J. Clin. Nutr. 46, 827–829.
Mitra AK, Alvarez JO, Guay-Woodford L, Fuchs GL, Wahed MA & Stephensen CB (1998): Urinary retinol excretion and kidney function in children with shigellosis. Am. J. Clin. Nutr. 68, 1095–1103.
Muto Y, Smith ES, Milch PO & Goodman DWS (1972): Regulation of retinol-binding protein metabolism by vitamin A status in the rat. J. Biol. Chem. 247, 2542–2550.
Olmedilla B, Granado F, Southon S, Wright AJA, Blanco I, Gil-Martinez E, Van Den Berg H, Corridan B, Roussel AM, Chopra M & Thurnham DI (2001): Serum concentrations of carotenoids and vitamins A, E, and C in control subjects from five European countries. Br. J. Nutr. 85, 227–238.
Pacheco-Santos LM, Dricot JM, Asciutti LS & Dricot-D’ans C (1983): Xerophtalmia in the state of Paraiba, northeast of Brazil: clinical findings. Am. J. Clin. Nutr. 38, 139–144.
Raghuramulu N, Underwood BA, Bhaskaram P, Arunjyothi, Chennaiah S & Reddy V (1998): Vitamin A relative dose response test in undernourished children. Nutr. Res. 18, 533–541.
Sommer A (1998): Xerophthalmia and vitamin A status. Prog. Retinal Eye Res. 17, 9–31.
Stephensen CB (2000): When does hyporetinolemia mean vitamin A deficiency? Am. J. Clin. Nutr. 72, 1–2.
Stephensen CB, Alvarez JO, Kohatsu J, Hardmeier R, Kennedy Jr JI & Gammon Jr RB (1994): Vitamin A is excreted in the urine during acute infection. Am. J. Clin. Nutr. 60, 388–392.
Tanumihardjo AS, Muhilal, Yuniar Y, Permaesih D, Sulaiman Z, Karyadi D & Olson JA (1990): Vitamin A status in preschool-age Indonesian children as assessed by the modified relative-dose–response assay. Am. J. Clin. Nutr. 52, 1068–1072.
Tarwotjo I, Tilden R, Pettiss S, Sommer A, Soebidjo S, Hussaini G, Sulistijo JS & Nendrawati H (1983): Interactions of community nutritional status and xerophthalmia in Indonesia. Am. J. Clin. Nutr. 37, 645–651.
Thurnham DI, McCabe GP, Northrop-Clewes CA & Nestel P (2002): A meta-analysis of data from 15 studies to quantify the effects of sub-clinical infection on plasma retinol. J. Nutr. 132 (Suppl), S2979 (abstract).
Tomkins A (2000): Malnutrition, morbidity and mortality in children and their mothers. Proc. Nutr. Soc. 59, 135–146.
Underwood BA (1998): Vitamin A Deficiency. Bull. World Health Organ. 76 (Suppl 2), S124–S125.
Underwood BA & Arthur P (1996): The contribution of vitamin A to public health. FASEB J. 10, 1040–1048.
Van den Berg H (1996): Vitamin A intake and status. Eur. J. Clin. Nutr. 50 (Suppl 3), S7–S12.
West Jr. KP (2002): Extent of vitamin A deficiency among preschool children and women of reproductive age. J. Nutr. 132 (Suppl), S2857–S2866.
Wolbach SB & Howe PR (1925): Tissue changes following deprivation of fat-soluble A vitamin. J. Exp. Med. 42, 753–777.
Wolf G & Phil D (1978): A historical note on the mode of administration of vitamin A for the cure of night blindness. Am. J. Clin. Nutr. 31, 290–292.
World Health Organization (1995): Physical Status: The use and Interpretation of Anthropometry, Report of a WHO Expert Committee, (Techical Reports Series No. 854), Geneva: WHO.
World Health Organization (1996): Indicators for assessing Vitamin A deficiency and their application in monitoring and evaluating intervention programmes: report of a joint. Geneva: WHO/UNICEF [Micronutrient series] WHO/NUT/96.10.
Acknowledgements
We thank Mrs Mônica Meirelles (Laboratory of Nutrology, Department of Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil and the staff of the Communitary Social Medical Center of Vila Lobato for their collaboration in this study, and Fundação de Apoio ao Ensino, Pesquisa and Assistência do Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto-USP (FAEPA) for the financial support.
Author information
Authors and Affiliations
Contributions
Guarantor: IS Ferraz
Contributors: ISF, JCD and HV were responsible for the design, the execution, analysis and writing of the paper. AAJ Jr., RGR, LADC, CEM Jr., AADE, LRCB were responsible for the execution, analysis and writing of the paper. HF was responsible for the analysis of the paper. All contributors approved the manuscript.
Corresponding author
Rights and permissions
About this article
Cite this article
Ferraz, I., Daneluzzi, J., Vannucchi, H. et al. Detection of vitamin A deficiency in Brazilian preschool children using the serum 30-day dose–response test. Eur J Clin Nutr 58, 1372–1377 (2004). https://doi.org/10.1038/sj.ejcn.1601978
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.ejcn.1601978
Keywords
This article is cited by
-
Impact of maternal vitamin A supplementation on the mother–infant pair in Brazil
European Journal of Clinical Nutrition (2010)
-
Vitamin A deficiency among Brazilian school-aged children in a healthy child service
European Journal of Clinical Nutrition (2009)