Abstract
THE yellow colour of human serum is generally assumed.to be caused mainly by bilirubin. The bilirubin concentration can be determined by means of the diazo reaction as modified by Jendrassik and Grof1,2. By the addition of pure bilirubin to serum, I was able to demonstrate that the proportion diazo reaction yellow colour in human serum varied within narrow limits ; if both are measured in the Pulfrich photometer, the diazo reaction with the filter S.611 and the yellow colour with S.433, the proportion between the extinctions (E.6l/E.43) varies between 1.38 and 1.60 (average 1.455) ; twenty-five human sera poor in bilirubin to which pure bilirubin was added were investigated4.
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WITH, T. OCCURRENCE IN HUMAN SERUM OF YELLOW SUBSTANCES DIFFERENT FROM BILIRUBIN. Nature 158, 310 (1946). https://doi.org/10.1038/158310a0
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DOI: https://doi.org/10.1038/158310a0
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