Abstract
Astwood and Jones1, and Talbot et al.2, have shown that not more than about 70 per cent of the theoretical amount of pregnanediol can be obtained from sodium pregnanediol glucuronidate after hydrolysis with hydrochloric acid. Using a colorimetric method of determining pregnanediol involving the development of a yellow colour on treatment with concentrated sulphuric acid, which is essentially similar to the method employed by Talbot et al., we have confirmed this finding. In the belief that the low yield of pregnanediol obtainable from the glucuronide was due to partial destruction in side-reactions during the hydrolysis, we decided to attempt the isolation and identification of the destruction products.
Similar content being viewed by others
Article PDF
References
Astwood, E. B., and Jones, G. E. S., J. Biol. Chem., 137, 397 (1941).
Talbot, N. B., Berman, R. A., Maclachlan, E. A., and Wolfe, J. K., J. Clin. Endocrin., 1, 668 (1941).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MARRIAN, G., GOUGH, N. Isolation of Pregnane-3(a)-ol-20-one from the Hydrolysis Products of 'Sodium Pregnanediol Glucuronidate'. Nature 157, 438 (1946). https://doi.org/10.1038/157438b0
Issue Date:
DOI: https://doi.org/10.1038/157438b0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.