Abstract
THE following observations were made during pilot investigations on the purification of thyrotrophin from acetone-dried ox pituitary. An aqueous alkaline (pH 9–10) extract of the dried gland (from which some inactive material had been removed by iso-electric precipitation at pH 5) was brought to pH 2 with salicylsulphonic acid. The thyrotrophic activity was divided between the precipitate and the supernatant fluid, each of which apparently contained 100–200 per cent of the original activity. This was shown in three separate experiments. The third experiment showed the least augmentation, which was unfortunate, as it was carried out to test the effects of dialysis (20 hr. through collodion) on the augmenting substance. The results, at least for the supernatant fluid, suggest that the augmenting substance is dialysable, though the small augmentation observed and the few animals used in the test do not establish this statistically.
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Biochem. J., 28, 1829 (1934).
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RINDERKNECHT, H., WILLIAMS, P. Augmentation of Thyrotrophic Activity. Nature 155, 606 (1945). https://doi.org/10.1038/155606a0
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DOI: https://doi.org/10.1038/155606a0
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