Abstract
IN the course of attempts to purify the corticotrophic hormone of the pituitary anterior lobe in the past few years, it was noted that the corticotrophic hormone shows far greater solubility in alcohol than other pituitary anterior lobe principles; it was also found to be more thermostable. Fresh pituitaries were therefore refluxed with ten times their weight of absolute ethyl alcohol (final alcohol concentration about 95 per cent) at pH 5. Such extracts gave a far higher yield of corticotrophic activity than any other extraction method previously employed; but the most interesting fact found in connexion with the hot alcoholic extraction of the glands was that, on being filtered and cooled, the extract deposited a considerable amount of solid matter which quickly settled. The corticotrophic activity of both the supernatant fluid and of the deposit were estimated by the method of Reiss et al.1. A considerable amount of corticotrophic activity was found in the fluid, but it was of particular interest that the acetone-dried deposit contained an unexpectedly high amount of corticotrophic hormone. Other anterior pituitary biological activity could not be found.
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Endokrin., 18, 1 (1936).
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REISS, M., GOLLA, Y. Purification of Anterior Pituitary Corticotrophic Hormone. Nature 155, 456 (1945). https://doi.org/10.1038/155456a0
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DOI: https://doi.org/10.1038/155456a0
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