Abstract
IN our investigations on the nutritional requirements of the larva of Aedes œgypti, our procedure has been based on the methods employed by Trager1. Various modifications have been introduced which make for increased accuracy of observation and clearer interpretation of the results. Thus larger groups of larvæ were used, each larva being placed in a separate tube and its stage of development noted every morning and evening. All liquid media were sterilized by Seitz filtration and the contents of the tubes tested for sterility at the conclusion of each experiment.
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References
Trager, W., Amer. J. Hyg., 22, 18 (1935).
Tracer, W., New Jersey Mosquito Extermination Assoc., Proc. Ann. Meeting, 29, 46 (1942). (Chem. Abstr., 37, 1517).
O'Dell, B. L., and Hogan, A. G., J. Biol. Chem., 149, 323 (1943).
Hutchings, B. L., Bohonos, N., and Peterson, W. H., J. Biol. Chem., 141, 521 (1941).
Briggs, G. M., jun., Luckey, T. D., Elvehjem, C. A., and Hart, E. B., J. Biol. Chem., 153, 423 (1944).
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Purrmann, R., Ann. Chem., 546, 98 (1940). (Chem. Abstr., 35, 2147.)
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GOLBERG, L., DE MEILLON, B. & LAVOIPIERRE, M. Relation of 'Folic Acid' to the Nutritional Requirements of the Mosquito Larva. Nature 154, 608–610 (1944). https://doi.org/10.1038/154608b0
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DOI: https://doi.org/10.1038/154608b0
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