Abstract
WE have postulated previously that the catalytic decomposition by catalase of hydrogen peroxide to molecular oxygen and water is accompanied by changes in the valency of catalase iron1. This supposition is strongly supported by spectroscopic and manometric experiments carried out on azide-catalase. As additional evidence in support of this view, we brought forward the results of experiments carried out in Barcroft differential manometers, showing that under certain conditions even the activity of free catalase is inhibited when oxygen is completely removed from the surrounding medium and replaced by pure nitrogen. Our manometric experiments were repeated by Weiss and Weil-Malherbe2 who, using Warburg manometers, failed to obtain this inhibition. Johnson and van Schouvenburg3 also failed to confirm our results using luminescent bacteria as indicators of decomposition of hydrogen peroxide by catalase in complete absence of oxygen. However, we repeated our experiments from time to time, using different enzyme preparations, and invariably confirmed our previous results. The failure by other workers to obtain similar inhibition was explained by us4, 5 as being due probably either to some difficulty in complete elimination of oxygen from Warburg manometers or to some defects in their enzyme preparations.
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Weiss, J., and Weil-Malherbe, H., NATURE, 144, 866 (1939).
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KEILIN, D., HARTREE, E. Decomposition of Hydrogen Peroxide by Catalase. Nature 152, 626 (1943). https://doi.org/10.1038/152626a0
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DOI: https://doi.org/10.1038/152626a0
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