THE procedure adopted for the cultivation of the Rous sarcoma in vitro in accordance with the usual method described by A. Fischer1 is as follows: A fragment of tumour is incubated in a medium consisting of chicken plasma and dilute embryonic extract. After a time the growing culture begins to digest the surrounding medium and also itself. Such a culture will die out if it is not provided with fresh tissue by means of subculture. The sarcoma cells infiltrate the added tissue fragment within which they remain for a time embedded. If normal tissue is regularly added, the sarcoma cells can be cultured over an unlimited period and will retain their malignancy.
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Arch. exp. Zellforschg., 19, 475 (1937).
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HALBERSTAEDTER, L., DOLJANSKI, L. Transformation in vitro of Cultures of Normal Cells Treated with Rous Sarcoma Agent into Sarcoma Cultures. Nature 143, 288 (1939). https://doi.org/10.1038/143288a0
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