Abstract
FROM the cell-free solution of B. fluorescens liquefaciens, which has a powerful aspartase effect1, we have isolated aspartase by adding acetic acid up to pH 4.6 at 0° C. The precipitate thereby formed (precipitation begins at pH 4.8) gives, when rapidly filtered and dissolved in M/15 phosphate buffer (pH 7), a solution which possesses considerable aspartase and also asparaginase effect. This solution has no fumarase effect, even in experiments of long duration.
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References
Virtanen and Tarnanen, Biochem. Z., 250, 193 (1932).
Gale, Biochem J., 32, 1583 (1938).
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VIRTANEN, A., ERKAMA, J. Enzymic Deamination of Aspartic Acid. Nature 142, 954 (1938). https://doi.org/10.1038/142954a0
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DOI: https://doi.org/10.1038/142954a0
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