Synthesis of Growth Factors by Rhizobium trifolii

Abstract

A STUDY of the growth factor requirements of Rhizobium trifolii has indicated that, under suitable conditions, the organisms are able to synthesize all the organic substances essential for growth from a synthetic carbohydrate - mineral salts medium of known composition. Although various extracts of plants and micro-organisms contain a heat-stable factor stimulative to growth, it is not required for successful continuous transfer of the organism in synthetic media. A base medium, claimed by Allison and Hoover1 to be incapable of supporting continued growth of the nodule organism in the absence of extracts of Azotobacter or other substances (termed ‘coenzyme R â), was found to be satisfactory after adjusting the oxidation-reduction potential and adding iron. Although the growth was not profuse in comparison with media which contain plant extracts, continuous transfer was possible.

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References

  1. 1

    Allison, F. E., and Hoover, S. R., J. Bact., 27, 561 (1934).

  2. 2

    West, P. M., and Wilson, P. W., Science, in the Press.

  3. 3

    Laird, D. G., and West, P. M., Canad. J. Res., in the Press.

  4. 4

    Nilsson, R., Bjälfve, G., and Burström, D., Naturwiss., 18, 284 (1938).

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WEST, P., WILSON, P. Synthesis of Growth Factors by Rhizobium trifolii. Nature 142, 397–398 (1938). https://doi.org/10.1038/142397b0

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