Abstract
WHEN a solution of hæmoglobin and ascorbic acid in alkaline phosphate or in phosphate buffer of pH. 7.6 is exposed to air at 20° for 48 hours, the solution shows an absorption band in the red (at about 674 mµ). On reduction by hyposulphite, this band is replaced by a strong band at 629 mµ1. By denaturation of the globin by alkali or pyridine, a hæmochromogen with an absorption band at 619 mµ is obtained. The latter is identical with the “a-pseudohæmoglobin” of Barkan and Schales2) ; in the preparation of these authors the denaturation is caused by the strong alkalinity of the cyanide solution.
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References
First observed by Lemberg, R., and Wyndham, R. A., J. and Proc. Boy. Soc. New South Wales, 70, 347 (1937).
Barkan, G., and Schales, O., Z. physiol. Chem., 248, 96 (1937).
Anderson, A. B., and Hart, P. D'A., J. Path. Bact., 39, 465 (1934); Edlbacher, S., and Segesser, A. v., Naturwiss., 25, 461, 557 (1937).
Lemberg, R., Biochem. J., 29, 1322 (1935).
Lemberg, R., Cortis-Jones, B., and Norrie, M., Biochem. J., 32, 149, 171 (1938).
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LEMBERG, R., LEGGE, J. & LOCKWOOD, W. A Hæmoglobin from Bile Pigment. Nature 142, 148–149 (1938). https://doi.org/10.1038/142148b0
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DOI: https://doi.org/10.1038/142148b0
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