Splitting of the Hæmocyanin Molecule by Ultra-sonic Waves

Abstract

EMULSIONS and colloidal solutions may be produced by means of ultra-sonic waves1,2. Compounds such as starch and gelatine are depolymerized3,4. It seemed, therefore, of interest to study the action of sonic waves on high-molecular proteins such as the hæmocyanins, the molecules of which are of colloidal size. In the investigation I used hæmocyanin from Helix pomatia, which has a molecular weight of 6,740,000, a sedimentation constant of 99 × 1013 and which, upon change of the pH, gives three dissociation fragments with the sedimentation constants 62, 16 and 12 × 1013 respectively (corresponding to ½, 1 8 and 116 molecules). These fragments may reversibly recombine to form the original molecule5,6,7.

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BROHULT, S. Splitting of the Hæmocyanin Molecule by Ultra-sonic Waves. Nature 140, 805 (1937). https://doi.org/10.1038/140805a0

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