Signs and symptoms that are characteristic for depression include changes in the setpoint of the hypothalamic-pituitary-adrenocortical (HPA) system, which in the majority of these patients result in altered regulation of corticotropin (ACTH) and cortisol secretory activity. More refined analysis of the HPA system revealed that corticosteroid receptor (CR) signaling is impaired in major depression, resulting among other changes, in increased production and secretion of corticotropin-releasing hormone (CRH, also frequently abbreviated CRF) in various brain regions postulated to be involved in the causality of depression. This article summarizes the clinical and preclinical data, supporting the concept that impaired CR signaling is a key mechanism in the pathogenesis of depression. Mouse genetics, allowing for selective inactivation of genes relevant for HPA regulation and molecular pharmacology, dissecting the intracellular cascade of CR signaling, are the most promising future research fields, suited for identifying genes predisposing to depression. Focusing on these two research lines may also allow to gain insight into understanding how current antidepressants work and further, how more specific targets for future antidepressant drugs can be identified.
FUNCTIONAL IMPAIRMENT OF CENTRAL CORTICOSTEROID RECEPTORS
Until now, the serendipitous discovery of antidepressants in the 1950s has profoundly inspired hypotheses of the pathogenesis of depression. The well-known pharmacological effects of antidepressants on presynaptic uptake transporters and degradating enzymes (i.e., MAO) of serotonin and norepinephrine has focused research on causality and treatment of depression on the metabolism of functional biogenic amines and the capacity of their respective receptors to alter intracellular signaling pathways that ultimately induce changes in gene activity. Elevated circulating levels of stress hormones among depressives were recognized even before antidepressants were discovered, but these changes were seen as epiphenomena, reflecting the stressful experience of depression, although M. Bleuler (1919) already demonstrated that hormones have diverse psychotropic effects and suggested hormone treatments as potential antidepressants. A vast amount of evidence has accumulated, that reject the view that altered stress hormone secretions in depression are epiphenomenal.
During the past decade, several research groups formulated a hypothesis relating aberrant stress hormone dysregulation to causality of depression and submitted that antidepressants may act through normalisation of these HPA changes (review: Holsboer and Barden 1996). This hypothesis was derived from the following clinical observations in depressives: 1) the number of ACTH and cortisol secretory pulses is increased which is also reflected in elevated urinary cortisol production rates (Rubin et al. 1987); 2) levels of CRH in the CSF are elevated (Nemeroff et al. 1984); 3) the number of CRH secreting neurons in limbic brain regions is increased (Raadsheer et al. 1994); and 4) the number of CRH binding sites in the frontal cortex is reduced secondary to increased CRH concentration (Nemeroff et al. 1988). These studies were complemented by many neuroendocrine function tests including the suppressibility of ACTH and corticosteroids by the synthetic glucocorticoid dexamethasone (dexamethasone suppression test, DST). The DST showed that a high proportion of patients with various affective disorders have elevated cortisol levels (Carroll 1982), thus escaping the suppressive effect of dexamethasone. After CRH was discovered and characterized by Vale and coworkers (1981) initial studies employing ovine or human CRH in depressives showed that the ACTH response after injection of this neuropeptide was decreased, suggesting desensitized pituitary CRH receptors due to homologous downregulation by hypersecreted CRH (Gold et al. 1986; Holsboer et al. 1986).
The most sensitive neuroendocrine function test to detect HPA dysregulation combines the DST and the CRH stimulation test (dex/CRH test) (von Bardeleben and Holsboer 1989, 1991; Heuser et al. 1994; Rybakowski and Twardowska 1999). In this test, patients are pretreated with a single low dose (1.5 mg) of dexamethasone at 23:00 h and receive intravenously 100 μg CRH at 15:00 h the following day. The amount of ACTH and cortisol subsequently released is much higher among depressives. In fact, Heuser and coworkers (1994) concluded from their studies that the sensitivity of this test (i.e., likelihood to differentiate between normal and pathological states) is above 80%, depending on age and gender. Whereas CRH-elicited ACTH response is blunted in depressives, dexamethasone pretreatment produces the opposite effect and paradoxically enhances ACTH release following CRH. Similarly, CRH-induced cortisol release is much higher in dexamethasone-pretreated patients than following a challenge with CRH alone. The interpretation of the above findings is as follows: dexamethasone, due to its low binding to corticosteroid binding globulin and its decreased access to the brain (Meijer et al. 1998), acts primarily at the pituitary to suppress ACTH. The subsequent decrease of cortisol and the failure of dexamethasone to compensate for the decreased cortisol levels in the nervous tissue creates a situation that is sensed by central regulatory elements of the HPA system as a partial and transient adrenalectomy. In response to this situation, the secretion of central neuropeptides which are capable of activating ACTH secretion—mainly CRH and vasopressin—is increased. Vasopressin is known to synergize with CRH, overriding dexamethasone suppression at human corticotrophs: When vasopressin is infused at a low rate into dexamethasone pretreated controls, concurrent infusion with CRH induces an ACTH and cortisol response which is similar to the hormone secretory profile of depressives receiving the combined dex/CRH-test but without simultaneous vasopressin treatment (von Bardeleben et al. 1985). This finding led us to postulate that hypothalamic vasopressin is increased in depressives (von Bardeleben and Holsboer 1989). A more recent study by Purba and coworkers (1996) reporting increased numbers of vasopressin-expressing neurons in the parvocellular part of the hypothalamic paraventricular nucleus of depressives ultimately confirmed this view.
All three tests (DST, CRH test, and dex/CRH test) have been frequently administered to depressed patients by several research groups. Serial DSTs during a variety of antidepressant drug treatment revealed that whenever cortisol suppression was inappropriate, i.e., above a certain threshold, normalization of the neuroendocrine dysregulation was necessary for clinical remission to become manifest. In addition, if post-dexamethasone plasma cortisol levels increased over time or remained elevated the likelihood for an unfavorable clinical course or nonresponse to treatment was high (Holsboer et al. 1982; Greden et al. 1983).
Blunted ACTH response to CRH and the normalization of elevated CRH in the CSF after antidepressant-induced clinical remission has also been reported; these findings confirm a close association between HPA dysregulation and depressive psychopathology (de Bellis et al. 1993). The combined dex/CRH test proved particularly useful as a predictor of increased risk for relapse (Holsboer et al. 1987; Holsboer-Trachsler et al. 1994; Heuser et al. 1996; Zobel et al. 1999).
In those patients where the neuroendocrine abnormality persisted, the risk of relapse or resistance to treatment was much higher. Together, all studies reported so far indicate that reinstatement of a “normal” HPA setpoint is an important prerequisite for clinical improvement and furthermore, if HPA abnormalities persist or become more pronounced during drug treatment the respective individual is at increased risk for relapse.
A study that administered different doses of dexamethasone prior to CRH showed that ACTH and cortisol suppression occurs at higher dexamethasone dosages in the depressives than in matched controls. This shift of the dose response curve to higher dexamethasone dosages corroborates the view that negative feedback mechanisms through glucocorticoid receptors (GR), to which dexamethasone binds, are impaired in depressives (Modell et al. 1997).
The consequences of impaired regulation of cortisol secretion are manifold, ranging from untoward effects in peripheral tissues (e.g., osteoporosis) to changes in the central nervous system. The latter are believed to comprise effects on morphology as well as on cognitive function. Studies by Sheline et al. (1996, 1998, 1999) and Bremner et al. (2000) suggested that recurrent major depression is associated with hippocampal volume loss and that the degree of this change is determined by the duration of the illness. Considering the findings that depressed patients are frequently hypercortisolemic and that the degree of hippocampal atrophy in aged humans correlates with the degree of plasma cortisol increase over time and the current basal cortisol levels (Lupien et al. 1998), it has been proposed that the neuroendocrine changes in depression may account for the changes in hippocampal size seen in this disease. Importantly, these reductions in hippocampal volume which are also seen in patients with schizophrenia—a disease not particularly associated with enduring hypercortisolemia—do not necessarily reflect cell death (Nelson et al. 1998). Moreover, in post-traumatic stress disorder, decreased hippocampal volume is associated with normal or even reduced plasma and urine glucocorticoid contents (Bremner et al. 1999).
Studies in rats and tree shrews showed that psychological stressors may also induce atrophy in hippocampal CA3 pyramidal neurons which involves reversible remodeling of apical dendrites, a process where elevated excitatory amino acids are believed to be a primary cause, possibly amplified by increased glucocorticoids (Watanabe et al. 1992; Gould et al. 1997; Magariños et al. 1996, 1997). That factors—other than glucocorticoids—account for stress-induced reversible or permanent morphological changes in the hippocampus has also been underscored by a study in nonhuman primates (Leverenz et al. 1999). Based on works by Landfield et al. (1981), Kerr et al. (1991), and Sapolsky (1992), the study by Leverenz et al. (1999) administered high doses of glucocorticoids to aged macaques for 12 months, however, no evidence for decreased hippocampal volume, subfield volumes, subfield neuronal density, and subfield total neuronal number emerged. This finding is in accordance with a report by Müller and coworkers (1998), who studied postmortem brains of patients with depression and of patients treated with various synthetic corticosteroids and failed to observe morphological changes and signs of cell death under these clinical conditions. In this context, it is important to note that there is evidence that primates, unlike rats, have a relative paucity in GR, but a high density in mineralocorticoid (MR) in the hippocampus (Sánchez et al. 2000). Since, unlike cortisol, synthetic corticosteroids bind only poorly to MRs, studies using synthetic glucocorticoids, e.g., dexamethasone, in humans produce a situation where MRs remain unliganded in this brain formation. If it holds true that also in humans MRs are predominating over GR in hippocampus, dexamethasone pretreatment would deprive this brain region from CR signaling.
As reviewed by McEwen and coworkers (1992), adrenal steroids can exert a manifold of effects sometimes opposing on the rat hippocampus, i.e., they can be protective as well as deleterious. In line with this view, studies by Hassan et al. (1996, 1999) and Almeida et al. (2000) showed that corticosterone and dexamethasone given at low dosages can exert opposite effects on hippocampal cell viability. It is yet unclear whether these opposing effects are due to differences in penetration of these two corticosteroids through the blood brain barrier or whether they are due to differences in relative occupation of GR and MR. It also seems important to recognize in future studies that neurochemical and neuroanatomical effects of stress hormones can only be studied to a limited extent by exogenous administration of corticosteroids. Producing stress-like plasma cortisol concentrations results in a variety of central changes including altered expression of CRH and neurotrophins, which in turn may exert neuroprotective and other behavioral effects (Behl et al. 1997).
Another issue that deserves attention in future clinical and preclinical studies relates to the difference in the effects mediated by GR and MR (reviews: Trapp and Holsboer 1996a; de Kloet et al. 1998). To dissect their respective effects on cognition, studies similar to that of Newcomer et al. (1999), but using selective MR and GR agonists and antagonists, are needed. This is of particular importance if the provocative study of Sánchez and coworkers (2000) that reported a relative absence of GR in the primate hippocampus is corroborated. Based on measurements of GR and MR mRNA, Seckl et al. (1991) showed that in human hippocampus both receptors are highly expressed, but also confirmed that differences between species exist regarding the subfield distribution of GR and MR.
In the past, much emphasis had been put on the possibility that neurochemical and neuroendocrine changes associated with depression account for the morphological changes observed in the CNS of these patients. A recent study by Rajkowska et al. (1999) found reductions in density and size of neurons and glial cells in the dorsolateral prefrontal cortex of depressed patients and raised the question whether the observed specific histopathological changes in major depression may be due to a genetic predisposition for cortical cell changes. Studies that help to resolve this question are awaited and they need to include studies on gene networks that are involved in early brain development.
The patient population which would be ideally suited to study shifted HPA setpoints are subjects with inherited glucocorticoid resistance. These patients may either have a polymorphism in the GR gene or other alterations in genes, whose products are involved in glucocorticoid signaling. As a consequence of the ubiquitous GR resistance, elevated ACTH and cortisol secretion occurs without leading to symptoms of Cushing's syndrome. Nonetheless, other disturbances emerge that are mediated by those elevated adrenocortical hormones that bind to other steroid receptors, such as MR or androgen receptors. Thus, subjects with detected familial GR resistance were diagnosed to have hypertension, and among females hirsutism, menstrual irregularities, and acne were prevalent (Lamberts et al. 1992, 1996). Because such symptoms are not apparent in depression and because of the episodic course of this disease, it seems unlikely at first sight that the pathology underlying depression involves a major GR gene mutation.
Recent data, however, suggest that relative glucocorticoid resistance caused by GR mutations may not be as infrequent as previously thought (Koper et al. 1997). Thus, it seems pertinent to study whether psychiatric syndromes are more prevalent in patients with GR resistance and also whether such mutations may occur among psychiatric patients with dysregulation of the HPA system. It is of note that the presence of polymorphisms or mutations in the GR cannot be automatically inferred from glucocorticoid resistance because structure or assembly of cellular components, such as chaperones or other transcription factors involved in hormone signaling, may also be defective. On the other hand, the episodic nature of depression does not reject the possibility of GR gene polymorphism since compensatory mechanisms, which help to maintain stress hormone homeostasis most of the time, may be present. One such mechanism may include interaction of polymorphic GR with chaperones, which are responsible for individual GR-binding properties and in cooperation with other transcription factors can determine the activation or repression of gene expression through ligand-activated GRs (see Section III). The capacity of the chaperone/transcription factor assembly to compensate for functional deficits due to allelic GR variants may fluctuate and become less effective whenever other counteracting non-genetic factors (e.g., intracellular signals induced by environmental stressors) are activated.
Munich Vulnerability Study
In this light, the data from the Munich Vulnerability Study are of interest as they show that subjects who never suffered from a psychiatric disorder, but belong to families with a high genetic load for depression may display abnormal responses to the dex/CRH test (see Figure 1). These abnormalities were found to be constant over time and those subjects showing these depression-like HPA changes were considered to be at risk for developing depression (Holsboer et al. 1995; Modell et al. 1998). The possibility that these HPA disturbances are acquired and rather reflect the stressful experience of having a family member suffering from a mood disorder than a genetic risk can be rejected, as only a fraction of about 20% of the probands show these alterations. Interestingly, this figure corresponds well with the calculated risk for developing depression in later life of high risk probands (Lauer et al. 1998). Whether it proves true that these individuals are indeed prone to develop depression is currently under investigation in longitudinal follow-up studies.
While it may well be that some of the individuals showing impaired corticosteroid receptor function and increased risk for depression do have a GR mutation, e.g., a single amino acid substitution in the ligand binding domain leading to reduced corticosteroid sensitivity, most genetic studies reported so far reject the GR gene as a possible locus of inherited pathology (Detera-Wadleigh et al. 1992; Morissette et al. 1999). It would not be surprising, however, if genetic studies of large kindreds would reveal that GR-regulated genes or genes whose products are involved in stress hormone signaling are contributing to genetic susceptibility for depression.
The CR hypothesis implies that intracellular signaling of adrenocortical steroids is impaired in specific areas of the brain, resulting in a number of changes in gene activity and neurotransmitter production involved in causality of depression. Particular emphasis is put on the interaction of hormone-activated GR and the effects on CRH, which is believed to be a key neuropeptide in the pathogenesis of depression and other stress-related disorders (reviews: Owens and Nemeroff 1991; Holsboer et al. 1992).
There is no uniform relationship, however, between activated GRs and CRH secretion, as CRH is differentially regulated by GR in different regions of the brain. In most parts of the hypothalamic paraventricular nuclei (PVN), glucocorticoids suppress CRH and vasopressin as they suppress proopiomelanocortin, the precursor of ACTH of the anterior pituitary (Erkut et al. 1998). In some brain areas that include the central amygdala expression (Schulkin et al. 1998) and those nuclei of the PVN that project to the spinal cord accounting for CRH content in the cerebrospinal fluid (Swanson and Simmons 1989), corticosteroids upregulate CRH gene expression. Positive feedback of glucocorticoids on CRH synthesis and secretion in many brain areas is life-sustaining as it keeps the organism responsive to acute stressors under conditions of chronic stress. Elevated CRH in the amygdala, for example, is pertinent to maintaining appropriate emotional responsivity, particularly if stress exposure endures (review: Gray and Bingaman 1996).
The effects of CRH are mediated through specific receptors of which two different subtypes (CRH-R1 and CRH-R2) have yet been identified (Chalmers et al. 1995). Their neuroanatomical distributions suggest that they mediate different effects. Recent studies using antisense probes directed against CRH-R1 and CRH-R2 mRNA supported this notion as they showed that only reduced CRH-R1 levels produce anxiolytic effects in stressed rats (Liebsch et al. 1999). Reports on mouse mutants where one of these receptors was genetically inactivated confirmed that CRH-R1 mediates anxiety-like behavior (Timpl et al. 1998; Smith et al. 1998). In contrast, two out of three studies where the CRH-R2 was invalidated noted increased anxiety-like behavior (Bale et al. 2000; Kishimoto et al. 2000; Coste et al. 2000) (see also Section II)
GRs are present in all rodent brain areas and are most abundant in the hypothalamus where they repress CRH and vasopressin gene activity. Most MRs in the brain are located in the hippocampus where they may be co-expressed with GR by many, but not all neurons. Hippocampal MRs are not selective for the prototypic mineralocorticoid aldosterone, but bind the glucocorticoid corticosterone (or cortisol in primates) with approximately tenfold higher affinity than GRs. This “nonselectivity” of brain MRs is determined by the fact that unlike in peripheral cells, the enzyme 11β-hydroxysteroid dehydrogenase (11βHSD) does not effectively exclude corticosterone or cortisol from MR-targets in the hippocampus. For example, in epithelial cells of the human kidney containing MR, this enzyme converts cortisol into cortisone, which binds only poorly to MR, allowing electrolyte homeostasis through aldosterone alone. In the hippocampus, a different 11βHSD isoform is present, which does not provide such selectivity (van Haarst et al. 1996a; Seckl 1997).
Because of the about tenfold higher affinity of “nonselective” hippocampal MRs for corticosterone, these receptors are already almost completely occupied at basal levels of corticosteroid secretion. On the other hand, hippocampal GRs are only occupied when corticosteroid levels increase under stress conditions or at the peak of the circadian rhythm of corticosteroid secretion. The coexistence of MRs activated at low corticosteroid concentrations, and of GRs activated only at high concentrations, allows the brain to differentially respond to the wide range of concentrations over which corticosteroids are secreted. These responses are extremely diverse and include steroid effects on cell membranes. At low concentrations, corticosterone maintains neuronal excitability which is a predominantly MR-governed effect, whereas at higher hormone concentrations this is opposed by increasing GR activation (Joëls and de Kloet 1992). Specifically, under basal conditions where most of the MRs, but only a fraction of GRs are occupied, Ca++ inward currents are small in hippocampal neurons; this results in a stable firing rate, thus contributing to the “proactive” role of corticosterone in maintaining homeostasis (review: de Kloet et al. 1998). Under stress, more GRs are activated and there is an associated increase in Ca++ influx and responsivity to serotonin (5-HT), for example, increases. This condition is referred to as the “reactive” mode by which corticosteroids protect neurons through GR and MR by reinstating homeostasis (de Kloet et al. 1998). Thus, it is the ratio of activated MR and GR, which determines not only the effects of corticosteroids on hippocampal neurons themselves but also in their projection areas, e.g., the amygdala.
The effects of the predominant amino acid transmitters in the brain, glutamate and γ-aminobutyric acid (GABA), which mediate excitatory and inhibitory effects on synaptic transmission respectively, are also controlled by the relative occupancy and activation of MR and GR. This is demonstrated by the modulatory effects of these two receptors on long-term potentiation (LTP), a phenomenon that refers to strengthening of synaptic contacts by repeated stimulation. When glutamatergic afferents to the hippocampus, particularly the CA1 area, are repeatedly stimulated, prolonged enhancement of synaptic responsivity is observed as a consequence. It has been suggested that changes in LTP correlate with the capacity to learn and retrieve memorized material. The induction of LTP has been found to be critically determined by the level of corticosteroids. As recently argued by de Kloet and coworkers (1998, 1999), this effect can be best explained by appreciating the specific roles of MRs and GRs and the ratio of ligand activated MR/GR on neuronal activity in the hippocampus. Under resting conditions, when corticosteroid levels are in the normal range, LTP is most pronounced and the MR/GR ratio is high because MRs but not GRs are fully occupied. This condition is associated with a facilitated adaptation to stressful situations where MR activation accounts for behavioral reactivity to acute stressors. Under chronic stress, a reduced MR/GR ratio results from increased GR occupation under increased corticosteroid levels and a gradual desensitization of MRs which precedes GR desensitization; the latter changes are associated with reduced LTP. It seems likely that such changes in synaptic efficiency represent the neural correlate of memory impairment associated with hypercortisolism. These cognitive deficits not only include disruption of memory consolidation but also impaired memory retrieval (de Quervain et al. 1998). In this context, it is important to note that such effects do not solely result from altered corticosteroid effects in the hippocampus. For example, GR-mediated memory consolidation is also influenced by β-adrenoceptor stimulation in the amygdala (Roozendaal et al. 1999).
The interaction between hippocampal GR and MR also plays a role in HPA regulation of rat. Studies using MR antagonists or MR antisense probes under trough (morning) or stress conditions resulted in exaggerated HPA activity (Oitzl et al. 1994; Reul et al. 1997). Interestingly, the GR antagonist mifepristone (RU 486, also blocking progesterone receptors) when injected intracerebroventricularly (icv) has no effect on basal trough levels because of low GR occupancy during early daytime hours. Administration of the same drug in the early evening, when corticosteroid levels are rising, leads to further HPA activity increase. However, if RU 486 is injected directly into the hippocampus, this results in a decrease of ACTH and corticosterone secretion (van Haarst et al. 1996b), indicating that hippocampal GRs, when activated by corticosterone binding, are opposing the inhibitory effects of MRs. In other words, MRs mediate an inhibitory tone on the HPA system which is opposed by activated hippocampal GR. Following icv administration, the excitatory effect of GR antagonism at the hypothalamus overrides the inhibitory effect of GR antagonism at the hippocampus. If the balance between MR and GR is intact, then hypothalamic and pituitary GR capacity is sufficient to maintain adequate feedback upon CRH neurons and corticotrophic cells. However, if corticosteroid signaling is defunct, stress-elicited HPA activity is gradually shifted towards operating at higher setpoints, resulting in continuous HPA hyperdrive with accompanying behavioral effects due to CRH and vasopressin disinhibition as well as the many other sequelae of MR/GRdysbalance.
The Nature-Nurture Conundrum
The risk of developing depression or other major affective disorders is determined by a complex interplay between genetic susceptibility, environmental exposures, and aging. These influences also account for long-term changes in the regulation of the stress hormone system. This is particularly well illustrated by animal studies in which stressors were administered pre- or postnatally, followed by evaluations of HPA function and emotionality during later life. From these experiments, it was concluded that context and timing are critical determinants for predicting whether early stress exposure results in hypo- or hyperactive HPA-status. For example, Reul and coworkers (1994) stressed pregnant female rats with immunostimulants and observed that their pups had increased activity throughout adulthood.
Since the original report by Levine and Mullins (1966), it has been repeatedly demonstrated that the effect of postnatal “handling”, i.e., a very brief daily separation of mother and pup results in reduction of both, emotionality and corticosterone secretion. Similarly, rats that had received normal maternal care (licking, grooming) during the first 10 days of their life had reduced plasma ACTH and corticosterone response to stress increased hippocampal GR and MR mRNA levels and decreased CRH mRNA when examined as adults. However, opposite effects were found, when these rats were severely traumatized postnatally by long mother-pup separations alone or in combination with mild foot shocks (Ladd et al. 1996). These rats showed increased CRH concentrations in the median eminence, decreased number of CRH receptors and pituitary ACTH-secreting cells, and hypercorticoidism, thus bearing resemblance to the neuroendocrine state found in depressives. Of special importance is the observation that rats that were postnatally traumatized perform poorly in learning and memory tests, as opposed to rats that had received optimal maternal care (Plotsky and Meaney 1993). These findings, which were also confirmed in experiments with non-human primates (Coplan et al. 1996), suggest that early trauma may persistently weaken corticosteroid signaling, leading to disinhibited release of hypothalamic CRH, ACTH, and corticosterone which, in turn, may have behavioral sequelae that are related to a number of depressive symptoms. These data might even be extrapolated to the human situation, explaining why individuals who were abused during childhood may be more likely to develop depression in later life (Heim et al. 2000).
A number of different experiments, however, demonstrates that early trauma does not necessarily result in HPA disinhibition and cognitive impairment. Oitzl et al. (unpublished results, cited in de Kloet et al., 1998) used a different strain of rats than the previous investigators and showed that in Brown Norway rats, which are known for their long and healthy life spans, early trauma (i.e., long mother-pup separation) resulted in a bimodal distribution of cognitive performance in later life. Among animals that were traumatized as pups, these investigators identified good as well as poor learners but only a few intermediate learners. In the control group, however, the majority of rats were intermediate learners and only few good or poor learners were observed. The maternally-deprived rats had decreased GR expression in the hypothalamus and in the hippocampus. Among these traumatized rats, the good performers had lower plasma ACTH and corticosterone concentrations as compared to the poor performers. These experiments point to strain-dependent effects and suggest that it is the individual genotype that determines the consequences of early trauma on the HPA-system and related behaviors. In view of this, the studies by Kendler and coworkers (1999) are important and have introduced an additional level of complexity as they show that the genetic endowment interacts with the environment; individuals with a high genetic risk for depression are more susceptible to the depressive episode-triggering effect of an adverse life event. In addition, genetic factors influence not only the sensitivity to potentially depressogenic life events, but also influence the likelihood to expose oneself to such an event. Hypothetically then, individuals with an inherited genetic trait that weakens stress hormone regulation through impaired corticosteroid receptor function may be more vulnerable to stressors and in addition they may select themselves into adversive situations which then trigger the onset of a depressive episode.
MOUSE GENETICS AS A TOOL TO PROBE THE CR HYPOTHESIS
Knock-out or Transgenic Manipulation of CRH and Corticosteroid Signaling
Mouse mutants with manipulations of the genes encoding either CRH or its receptors or CRs have been generated. As predicted from studies where CRH was injected into rodent brains, overexpression of CRH in mice results in an enhancement of anxiety-like behavior (Stenzel-Poore et al. 1994). Mice with deletions of the CRH gene exhibit normal stress-induced behavior, confirming that CRH may not be the only physiologically active ligand of CRH receptors. If CRH is absent, other neuropeptides such as urocortin or other yet-to-be identified molecules acting at CRH1 or CRH2 receptors (and possibly CRH receptors yet-to-be discovered) can serve as anxiogenic or depressogenic signals (Weninger et al. 1999).
Studies by Liebsch et al. (1995, 1999, Heinrichs et al. (1997), and Skutella et al. (1998), who used antisense probes directed against the mRNA-encoding CRH-R1 and CRH-R2 in rats, and also studies involving mouse mutants lacking CRH-R1, suggested that CRH-R1 mediates anxiety-like behavior (Timpl et al. 1998; Smith et al. 1998) (Figure 2). In contrast, CRH-R2 deficiency was shown to increase anxiety-like behavior in some (Bale et al. 2000; Kishimoto et al. 2000) but not all (Coste et al. 2000) studies, raising the possibility that the two so far identified CRH receptors mediate opposite effects on anxiety-like behavior. Recently, Radulovic and coworkers (1999) confirmed that the role of CRH in enhancing learning and precipitating anxiety-like behavior is brain area- and receptor type-dependent and that previous stressful experience can also modulate the CRH-mediated behaviors. CRH signaling through CRH receptors is further complicated because CRH is bound to CRH-binding protein (CRH-BP) which has an affinity for CRH that is equal to or greater than the respective CRH receptors (Potter et al. 1991). This 37-kDA protein binds 40–90% of total CRH and is present in most human brain regions at tenfold higher concentrations than total CRH, which points to a major role of CRH-BP as regulator of CRH, “buffering” its actions under conditions of excessive release (Behan et al. 1995).
Compounds that displace CRH from this carrier in the brain produce anxiety-like and anorectic behavior, which is similar to the behavioral phenotype of mice where the gene for CRH-BP had been deleted (Karolyi et al. 1999). The loss of appetite seen in these animals is plausibly explained by the increased “free” CRH and urocortin levels since both of these peptides exert anorectic effects through CRH-R1 and/or CRH-R2. The behavioral role of urocortin still needs to be clarified. This neuropeptide binds with higher affinity to both CRH receptors and this may explain why anxiety-like responses to stressors in CRH null mutant mice are indistinguishable from wild-type mice (Weninger et al. 1999). In the absence of CRH, urocortin may bind to CRH-R1 compensating the loss of CRH. Consistent with the notion that CRH and CRH receptors are involved in depression, anxiety and other stress-related conditions, transgenic mice which overexpress CRH-BP show decreased anxiety-like behavior and enhanced locomotor activity (Burrows et al. 1998).
The above mentioned studies confirmed the central role of CRH and its receptors in mediating stress-related hormonal and behavioral responses and are complemented by recent studies in mice with targeted mutations of corticosteroid receptors. In order to gain further insight into the causality of HPA aberrancies, the GR gene was disrupted, which resulted in a phenotype with 90–95% lethality (Cole 1996). Survivors had excessive ACTH and corticosterone levels and almost absent adrenal medullas. In these mutants, Meijer and coworkers (1997) showed that the GR deficit results in impaired processing of spatial information. Because this behavior had previously been attributed to MR, these authors suggested that MR effects in the hippocampus require intact GR function which is consistent with a role for GR/MR heterodimers in mediating these effects (Trapp et al. 1994). The fact that only a minor fraction of litters survives the disruption of GR suggests that these animals were the few that could successfully compensate GR deficiency, which has profound peripheral effects.
The true nature of these complex compensatory mechanisms is not clear and it will be difficult to identify their specific involvement in the observed behavioral phenotype. Therefore, the recently developed mouse mutants where the GR knockout was restricted to neural tissue using the Cre/LoxP-recombination system should be more promising for studies of the effects of GR disruption in the brain (Tronche et al. 1999). In these mice, exon 3, encoding the first zinc-finger GR DNA binding domain, was flanked by LoxP sites. Crossing these mice with mice that express the nestin-Cre transgene resulted in animals where GR protein was absent only in neurons and glia cells. In these mice, CRH was elevated in the hypothalamus and ACTH and corticosterone were hypersecreted indicating that ligand-activated pituitary GRs (which remained intact) are unable to fully compensate for effects of hypersecreted CRH through suppression of POMC gene expression. These mice also showed signs of reduced anxiety and impaired stress response suggesting that loss of corticosteroid signaling through GR in the brain has direct consequences for emotional behavior (Tronche et al. 1999).
These initial studies did not address whether GR deletion leads to reduced CRH gene expression in the central amygdala. In this brain region, CRH is believed to be enhanced by activated GR and the absence of neuronal GR function could plausibly result in decreased anxiety-like behavior (Schulkin et al. 1998). Notably, even cell-specific gene disruption does not resolve the issue of compensatory mechanisms which are perhaps also reflected in the surprising dissociation of ACTH and corticosterone secretion in these mice. Given the specific role of hippocampal MR in behavior and neuroendocrine regulation, it would be of great interest to study a mouse mutant where the MR gene deletion is restricted to the hippocampus. The MR knockout mice obtained by gene targeting that is not tissue specific, died between postnatal day 8 and day 13 due to massive sodium loss and subsequent electrolyte disturbance (Berger et al. 1998). Studies using techniques to inactivate MR selectively in the hippocampal formation are now warranted to better understand the role of this receptor, which in non-human primates is believed to be the predominant corticosteroid receptor in this brain region (Sánchez et al. 2000).
Transgenic Mice Expressing GR Antisense
Pepin and coworkers (1992) generated a transgenic mouse in which an 1815 base pair fragment of the 3′ non-coding region of the GRcDNA, downstream from a 2.3 kb Eco R1/Hind III human neurofilament promoter element, was inserted into the mouse genome. This resulted in a mouse expressing GR antisense mainly in neuronal tissue and this mutant was expected to be a well-suited animal model of depression associated with impaired GR function (Pepin et al. 1992). This transgenic mouse was extensively studied and the main findings that emerged were the following: 1) these mice needed higher dexamethasone dosages than control mice in order to display corticosterone suppression under basal conditions or following CRH (Stec et al. 1994); 2) CRH-elicited ACTH was higher in transgenic mice but corticosterone was lower in comparison to controls (Barden et al. 1997); 3) these mice showed decreased corticosterone response to exogenous ACTH (Barden et al. 1997); 4) when stressed, these mice showed increased ACTH levels, whereas corticosterone levels remain unchanged (Karanth et al. 1997); 5) Dijkstra et al. (1998) showed reduced activity of CRH neurons in the PVN of these mice and decreased sensitivity of pituitary CRH-R1 mRNA to stimulus-induced desensitisation; 6) these mice displayed an enhanced locomotor-stimulating effect to morphine, a response that is reflected by an enhanced dopaminergic activity within the mesolimbic system (Spanagel et al. 1996); 7) in these mice, responses to endotoxin were aberrant as noted by Linthorst and coworkers (1999), confirming that immune function is critically determined by appropriate GR function; 8) several studies (e.g., Montkowski et al. 1995; Rousse et al. 1997; Rochford et al. 1997; Ströhle et al. 1998) showed that these mice have impairments in learning and memory paradigms which are also influenced by age; 9) Steckler et al. (1999) concluded that allocentric spatial navigation is impaired whereas egocentric navigation is unimpaired. The latter authors suggested that the observed effects were due to hippocampal dysfunction secondary to GR deficiency and possible compensatory changes. From their study follows that the observed deficit is related to a general impairment in storage or retrieval of information; 10) Steckler and coworkers (2000a) further concluded that the behavioral phenotype of GR-impaired transgenic mice is characterized by altered motivation and enhanced impulsive responding rather than from mnemonic deficiency; 11) Linthorst et al. (2000) studied putative disturbances at the synaptic level in these mice employing an in vivo microdialysis probe. In transgenic mice, serotonin release was higher than in control mice when exposed to rats, usually perceived as a profound stressor by mice. Paradoxically, transgenic mice did not show typical stress-like behaviors (e.g., freezing), but exhibited arousal behavior (e.g., exploration and approach toward a rat). The exaggerated serotonin release in these mice perhaps reflects the reciprocal interaction between CRH and raphe-hippocampal serotonin activity. Chronically elevated levels of central CRH have been shown to cause hyporesponsiveness of hippocampal serotonin following an acute stressful stimulus (Linthorst et al. 1997). In these transgenic mice, hypothalamic release of CRH is low, possibly explaining the enhanced serotonin release (Dijkstra et al. 1998).
Critical appreciation of the herein referred to studies using mouse mutants allows one to conclude that manipulations of the expression of CRH and its receptors have largely confirmed what was expected from pharmacological experiments. Alteration of GR gene expression (knockout or antisense expression) has led to several unexpected findings, e.g., reduced anxiety-like behavior in mice with GR gene disruption in the CNS and decreased CRH expression in hypothalami of mice expressing GR antisense. It is of note that none of the mouse mutants generated so far, can be viewed as animal model of a specific psychiatric disease defined by common diagnostic procedures. However, as discussed below these mouse mutants seem to be of great value to study several selected symptoms such as anxiety, abnormal stress response, cognition, withdrawal from drugs of abuse, appetite, reproductive behavior, sleep, etc., that are associated with HPA disturbances.
MOLECULAR MECHANISMS OF CORTICOSTEROID SIGNALING
The translation of the corticosteroid signal into a cellular response can be dissected into three steps: 1) entry of corticosteroids into the cell which occurs by passive transmembrane passage; 2) binding to corticosteroid receptors whose binding capacity is determined by chaperone-assisted folding; 3) trafficking to the nucleus that involves dissociation of chaperones and cooperative actions of transporters; and 4) nuclear actions of ligand-activated receptors either through DNA binding at specific response elements (GRE) or through protein-protein interaction with other transcription factors.
The first step in corticosteroid signaling, its passage through the cell membrane, is regarded as a passive process due to the lipophilic nature of steroid molecules. Because the affinity of steroid molecules for intracellular receptors is higher than for extracellular transport proteins (corticosteroid-binding globulin), there is enrichment of corticosteroid molecules within target cells. Only recently, specific interactions of corticosteroids with molecules residing within the cell membrane have been identified. From studies with ion channels composed of GABAA receptor subunits, evidence emerged that so-called neuroactive steroids (usually reduced at the ring A of the steroid molecule) interact by modulating chloride ion conductance (reviews: Paul and Purdy 1992; Rupprecht and Holsboer 1999).
In non-mammalian cells, Orchinik and coworkers (1991) found preliminary evidence for a corticosterone receptor-like structure in synaptic membranes and others (Alléra and Wildt 1992; Lackner et al. 1998) suggested the existence of a glucocorticoid carrier within the membrane of rat liver cells. It is yet unresolved whether such mechanisms also exist in neurons. These studies need to be substantiated in various tissues before the possibility of membrane-mediated actions can be recognised as crucial step in steroid signaling. That the access of corticosteroids to nerve cells can also be limited by an active process has recently been shown by Schinkel et al. (1995). Uptake of moderate amounts of dexamethasone was found to be limited by a multidrug resistance gene product, a P-glycoprotein extrusion pump that actively exports synthetic corticosteroids, but not naturally occurring hormones out of the cell. Mice lacking this gene have a higher central uptake of some synthetic neuroactive compounds (Uhr et al. 2000) including dexamethasone (Meijer et al. 1998).
When entering the cell, the corticosteroid molecule binds to MR or GR which are part of a dynamic multiprotein complex, composed of the steroid receptor and an array of chaperones which include heat shock proteins. Chaperones function as assembly systems for steroid receptors as well as other protein molecules involved in corticosteroid signal transduction. The basic assembly system (termed “foldosome”) has been reconstituted in vitro and was shown to require hsp90, hsp70, p60/hop (hsp70-organizing protein) and hsp40 (Pratt and Dittmar 1998). The foldosome initially associates with the nonsteroid binding state of the receptor. Under in vivo conditions the foldosome may require additional proteins, e.g., hip (hsp70-interacting protein). In the final heterocomplex, hop is replaced by immunophilins, e.g., FKBP 51 (also termed FKBP 59) or FKBP 52 (also termed FKBP 59, p59, or hsp56). This heterocomplex is further stabilized by p23, a protein that binds directly to hsp90 and maintains the corticosteroid receptor in a conformation that facilitates hormone binding. It is of note that the stepwise assembly and conformational maturation of the heterocomplex requires energy input through ATP. Both chaperones, hsp90 and hsp70, are ATP-binding and -hydrolyzing proteins. The role for hsp90 and the importance of ATP to maintain receptor conformation has been studied using the benzoquinone geldanamycin, which occupies the nucleotide binding site on hsp90 and prevents the switch to its ATP-bound conformation. Through this inhibition, the corticosteroid receptor is prevented from being assembled into the heterocomplex and is kept in a low affinity state for its ligands and steroid receptor-induced transactivation is therefore abolished.
Another example how chaperoning determines CR signaling is that the ratio between hsp70 and a protein that binds hsp70 as a co-chaperone, termed BAG-1 (Bcl-2-associated gene product-1) modulates the glucocorticoid-binding activity of the GR hsp90 heterocomplex, as increasing BAG-1 inhibits GR folding and thus decreases corticosteroid signaling (Kanelakis et al. 1999; Nollen et al. 2000). From these findings, it can be concluded that changes in the chaperone assembly can have a manifold of effects on activation and repression of GRE-regulated genes. The relevance of stoichiometric changes in chaperone expression has been demonstrated in the squirrel monkey, a species with excessively high cortisol levels, but no signs of Cushing's syndrome (Chrousos et al. 1982). This discrepancy is currently best explained by a markedly decreased binding affinity of GR, due to an overexpression of immunophilin FKBP 51 in squirrel monkey (Reynolds et al. 1999).
Once the steroid is bound, it induces a conformational change in the receptor and subsequently heat shock proteins and immunophilins dissociate, allowing for nuclear actions of activated MRs and GRs. This dissociation equilibrium is a dynamic process and GR and MR can be recycled by reassociating with chaperones. There is increasing evidence that (co)chaperones are not only important for GR activity in the cytosol, but also in the nucleus. For example, hsp90 may participate in the nuclear-cytoplasmic shuttling of GR (Kang et al. 1999) and it may also facilitate chromatin recycling of GR (Liu and DeFranco 1999). More recently, the cochaperone RAP 46 (also known as HAP 46 or BAG-IL) which can associate with hsp90 and GR has been identified as a non-specific DNA-binding protein which may act as a general transcriptional activator (Zeiner et al. 1999), on the other hand, Schneikert and coworkers (1999) reported that RAP 46 downregulates GR-mediated transactivation, but not transrepression. Altogether, it becomes clear from these few selected examples that future research linking HPA function with causality of depression and course of treatment needs to include studies on the role of chaperones in GR signaling.
Ligand-activated GRs and MRs can either activate or deactivate expression of target genes. Activation occurs through formation of GR-GR and MR-MR homodimers or GR-MR heterodimers, which bind to short palindromic DNA sequences called glucocorticoid response elements (GRE, see above) in the promoter region of corticosteroid-responsive target genes. Whether the response elements to which receptor homodimers or heterodimers can bind are all identical is not yet known. The role of activated GR and MR functioning as nuclear transcription factors is to recruit various factors that are able to remodel the chromatin structure at the promoter of the target gene and to recruit and maintain a transcriptional pre-initiation complex. A fine-tuned mechanism orchestrates gene activation through GRE and only a few aspects highlighting possible causes of impaired GR and MR function at this level and consequences for potential targets are briefly discussed (reviews: Beato et al. 1995; Mangelsdorf et al. 1995; Shibata et al. 1997; Freedman 1999) (see Figure 3).
When bound to DNA, the steroid receptor associates with coactivators, such as steroid receptor coactivator 1 (SRC-1, member of the p160 family). If SRC-1 is coexpressed in an assay system analysing GRE-regulated gene expression (such as the mouse mammary tumor virus, MMTV) then the transcriptional efficacy of GRs is strongly enhanced. The situation is even more complex as the enhancement of GR by the coactivator SRC-1 is amplified by CBP, a protein that binds CREB (cyclo AMP response element binding protein). This synergy may function through more efficient recruitment of basic (”general”) transcription factors (GTF) and stabilization of the preinitiation complex, thus enhancing mRNA synthesis by polymerase II. SRC-1 and CBP are only two of the many recently discovered coactivators of GR-mediated transcriptional activity and the mechanism by which they drive transcription is only poorly understood. One such mechanism is the acetylation of histones by SRC-1 which results in localized chromatin remodelling and assembly of the basal transcription machinery into a stable preinitiation complex (Spencer et al. 1997). While all coactivators identified so far are proteins, a recent study by Lanz and coworkers (1999) identified an RNA molecule that fulfills all characteristics of a coactivator of SRC-1. This RNA transcript called SRA (steroid receptor RNA activator), if co-expressed in assays of steroid receptor-mediated transactivation, can induce a tenfold enhancement of receptor gene activity, which can be abolished when SRA antisense oligodeoxynucleotides are added to the cells. It is not clear whether modulation of GR mediated transactivation by RNA molecules is a physiologically relevant mechanism. This theoretical possibility, however, opens up many new opportunities to interfere with glucocorticoid actions in the nucleus.
Reduction of gene expression by glucocorticoid receptors can be achieved through DNA-bound GRs as well as by interference of GRs with those nuclear transcription factors, that otherwise enhance gene activity. The physiological role of response elements, which reduce transcription when GR dimers are bound, is still debated. Only in a few cases, for example, the negative GRE-mediated regulation of the proopiomelanocortin gene expression, a mechanism requiring DNA-binding receptor dimers, seems to be effective (Drouin et al. 1993). Another example is that of the recently localized cis-acting site of the human CRH promoter that mediates negative regulation by ligand-activated GR (CRHnGRE) (Malkoski et al. 1997). Under the experimental conditions used (transient expression in mouse corticotroph-derived AtT-20 cells), the CRHnGRE, however, can be activated by cAMP or CREB (Malkoski and Dorin 1999).
To further appreciate these findings in the context of the CR hypothesis, it would be desirable to reproduce these findings under conditions that allow extrapolations on the in vivo condition. Still another possibility of GR-induced reduction of gene activity is that some molecules associated with GRE bound receptors act as corepressors (see Figure 3). Such a functional interaction between a latent transcription factor called Stat5 (signal transducer and activator of transcription) and the GR has been shown (Stoecklin et al. 1996). The GR can form a physical complex with Stat5, which decreases its DNA binding and thus GR-driven transactivation. This effect, first demonstrated in the mouse mammary tumor virus (MMTV) promoter (which contains four copies of the GRE), required phosphorylation and dimerisation of Stat5. The MR is similarly affected by Stat5, however, at a lower rate (Stoecklin et al. 1997).
The best studied example of how corticosteroid receptors negatively regulate gene transcription through protein-protein binding is a group of negatively GR-regulated genes that contain an AP-1 (activating protein 1) binding site in the promoter. AP-1 is a dimer composed of Jun and Fos (review: Pfahl 1993). If GR is ligand-activated it interacts as a monomer with the Jun/Fos dimer resulting in decreased AP-1 driven transcription (Figure 3). An example where both mechanisms (transactivation through genomic action and transrepression through protein-protein interaction) occur simultaneously is the effect of ligand-activated GR on the activity of NFκB (nuclear factor κB), a factor which is induced by many stimuli, predominantly those related to inflammation. In its inactive form NFκB (composed of a p50-p65 heterodimer, although other members of the RelA family can also constitute this transcription factor) is complexed by the inhibitory IκB subunit. When activated by corticosteroids, GRs can activate IκB expression through a GRE in the IκB gene promoter (Auphan et al. 1995). Furthermore, activated GRs interact with p65 and may therefore prevent p50-p65 binding to κB sites in the promoters of cytokines or cell adhesion factors which mediate inflammatory response (Caldenhoven et al. 1995). This dual action of corticosteroids through GR-protein interaction and gene activation works concurrently to suppress clinical symptoms associated with inflammation (Wissink et al. 1998; van der Burg et al. 1997).
Another mechanism which may reduce the efficiency of GR induced gene activation is altered splicing of GR pre-mRNA, resulting in overexpression of the GRβ variant that is devoid of transactivity. Heterodimerisation of GRβ with the transactive GRα results in diminished GRE regulated gene activity (Bamberger et al. 1995). A nuclear orphan receptor has also been identified that represses GR mediated transcriptional activity (Trapp and Holsboer 1996b). Other orphan nuclear receptors that interfere with GR and are directly involved in HPA activity belong to the nurr1/nur77 subfamily. These molecules do not require ligand binding and can confer transcriptional activity through specific response elements without ligand activation (Drouin et al. 1998; Murphy and Conneely 1997). Nur77, which transactivates either as a monomer or as a dimer through specific response elements, is of particular importance for the maintenance of basal ACTH secretion. Only during stress, when CRH is released from the hypothalamus reaching the anterior pituitary through portal vessels, CRH-R1 receptor-mediated enhancement of proopiomelaonocortin gene expression and subsequent increased secretion of ACTH from corticotrophs occurs.
While most experiments elucidating cellular effects of ligand-activated corticosteroid receptors have been conducted in artificial cell systems, some of the conclusions drawn so far have been corroborated in a mouse mutant where a specific point mutation was introduced into the GR (Reichardt et al. 1998). These authors mutated the GR gene by exchanging alanine and threonine, in position 458. This was based on a study by Heck and coworkers (1994) who observed that this mutation, which is located in the second zinc-finger in the DNA binding domain of the GR, abolishes receptor dimerisation and thus DNA binding. In mice homozygous for this point mutation, CRH expression was unimpaired, whereas POMCmRNA and ACTH were elevated. This finding is consistent with a negative regulation of the CRH gene through GR monomers that do not bind to DNA but transrepress through protein-protein interaction. Alternatively, the activation of a negative GRE in the CRH promoter may not require GR dimerisation (Malkoski and Dorin 1999). In contrast, pituitary corticotrophs require GR dimerisation which is in accord with DNA binding at a negative response element or interaction as a dimer with nur77 (Philips et al. 1997).
The examples referred to in this section show that a great number of possibilities exist for changing the setpoint of an individuals HPA activity. It is important to note that such setpoints are variable across individuals, but fairly stable intra-individually (Huizenga et al. 1998). This is even true among individuals with an increased genetic risk for depression where alterations of this setpoint are believed to be causally related to pathogenesis (Modell et al. 1998).
CONSEQUENCES FOR FUTURE DRUG TREATMENT OF DEPRESSION
Antidepressants are clinically effective not only in depression and panic disorder, but also in generalized anxiety and social phobia, and constitute one of the most successful treatment modalities in medicine. In contrast to this clinical situation, elucidation of the mechanisms of action of these drugs has been less successful. The hypothesis that has dominated the psychopharmacology of depression for decades is based on the assumption that a biogenic amine-deficiency underlies mood disorders and that this can be remedied by inhibition of presynaptic reuptake transporters of serotonin (synthesized and released from dosal raphe nucleus) and/or norepinephrine (originating from the locus coeruleus), thus increasing the transmitter concentration at postsynaptic sites. This hypothesis does not explain why it takes many weeks, often months, before antidepressants become effective, whereas reuptake inhibition occurs immediately.
A refinement of the monoamine hypothesis was forwarded by studies that depleted central serotonin and norepinephrine bioavailability. These studies found that normal individuals do not develop depressive symptoms when serotonin or norepinephrine is depleted through specific diets (Delgado et al. 1990). It was found, however, that patients having previously responded to a selective serotonin reuptake inhibitor (SSRI) worsen clinically when experimentally deprived from serotonin, but not from norepinephrine. In turn, those patients having responded to a norepinephrine reuptake inhibitor had increased depressive symptomatology when norepinephrine depletion was induced through diet with methyl para-tyrosine (Delgado et al. 1992). It was concluded from these studies that antidepressants rather than acting through a specific pharmacological mechanism may trigger a long cascade of events which then converge to act through a final common pathway. The view that the primary effect of antidepressant-induced changes on uptake transporters is remote from the therapeutically-relevant action is also fueled by a recent study that failed to find a difference in the clinical efficacy of the selective serotonin reuptake inhibitor paroxetine when compared to tianeptine, which enhances serotonin reuptake (Nickel et al., unpublished results), which is in accord with results of a study that compared tianeptine with fluoxetine (Lôo et al. 1999).
Two new hypotheses, which are complementary rather than mutually exclusive, have been forwarded to explain how antidepressants work at the neurobiological level. One hypothesis, developed by Duman et al. (1997), focusses on the effects of activation of the cAMP cascade through cell membrane receptors, followed by enhanced induction of CREB and hippocampal brain derived neurotrophic factor (BDNF). The other hypothesis, which is the fundament of this treatise, submits that antidepressants act through improving CR function. In the CNS, these modulations also affect brain regions not or only indirectly connected to the peripheral HPA system, which regularly stabilizes under the influence of antidepressants.
Studies supporting the cAMP/CREB/BDNF-hypothesis documented that the expression of CREB is enhanced by antidepressants and electroconvulsive treatments. In addition, the stress-induced decrease of BDNF is blocked by antidepressants. Because the BDNF gene contains a cAMP response element (CRE) to which phosphorylated CREB (P-CREB) binds and enhances transcription, it is assumed that activating cAMP through increased amine binding at G protein-coupled cell membrane receptors will ultimately result in increased expression of BDNF. This neurotrophic factor, when injected centrally in high dosages to rats produces changes in behavior that are reminiscent of changes induced by antidepressants (Siuciak et al. 1996). The antidepressant-induced expression of BDNF would also explain the beneficial effect of these drugs upon neuronal survival and growth of hippocampal neurons which are believed to be potentially endangered as a consequence of the neuroendocrine changes during a depressive episode (see Section I).
As the authors of this hypothesis point out themselves, CREB and BDNF regulation are not the sole targets of antidepressants (review: Duman et al. 1997). Their hypothesis rather exemplifies a new approach in exploring how antidepressants may exert their therapeutic of action beyond the receptor level. More specifically, studies related to the cAMP/CREB/BDNF-hypothesis will prove whether the absolute amount of CREB, increased by antidepressants is relevant for transactivation of CRE-regulated genes. In the future, the degree of phosphorylation of CREB (P-CREB) following acute and long-term treatments with antidepressants needs to be analysed, because only P-CREB can bind to DNA, thereby accounting for CRE-induced transactivation. Such studies are pertinent since Rossby and coworkers (1999) examined the long-term effects of venlafaxine, which inhibits both, the serotonin and the norepinephrine reuptake transporter. These authors reported that CREB expression was unchanged by venlafaxine, whereas the transcriptionally active phosphorylated P-CREB was reduced in the cortex of rats. Because neither endogenous nor cAMP-stimulated protein kinase A (PKA) activity was changed by venlafaxine, the decrease of P-CREB was not attributed to decreased cAMP. If this venlafaxine-induced decrease in CREB phosphorylation is also effective in the hippocampus, BDNF expression would be expected to be decreased by this drug in this brain area. This would be not consistent with a general enhancement of BDNF expression by effective antidepressants.
The important information obtained from these studies is that antidepressants exert nuclear effects that are not necessarily mediated by the well-characterized signaling pathways. This seems particularly true when one tries to unify the CR hypothesis and the cAMP/CREB/BDNF hypothesis. First, there is substantial cross-talk between CR signaling and CREB phosphorylation. GR does not prevent CREB binding at DNA but physically associates with CREB and thus decreases phosphorylation of CREB. Provided that the observation of Rossby and coworkers (1999) is not limited to the specific effects of venlafaxine in the cortex, then decreased P-CREB should result in decreased expression of CRH, which contains a CRE in its promoter (Seasholtz et al. 1988; Spengler et al. 1992). Legradi and coworkers (1997) observed that glucocorticoids can abolish CREB phosphorylation in CRH neurons. Thus, if GR signaling is impaired, CRH expression through P-CREB is enhanced and this effect is counteracted by venlafaxine (and perhaps also by other antidepressants) which reduces P-CREB (Rossby et al. 1999). Still, other impairments in cAMP-PKA pathways may possibly exist in depression (Shelton et al. 1999), including impaired CRE-regulated gene expression which can be restored by antidepressants. Since cross-talk between cAMP-PKA pathways and corticosteroid signaling is well documented, impaired corticosteroid receptor function could not only result from altered cAMP-PKA activity but may in turn account for disturbances in cAMP-PKA-elicited activation of CRE.
The second observation that allows both hypothesis to converge is that postreceptor effects of antidepressants do not necessarily implicate cell membrane receptor G protein cAMP-PKA pathways or ion channel-mediated activation of kinases. Inasmuch as BDNF can be regulated by other mechanisms than cAMP-CREB, corticosteroids can also be functionally regulated by antidepressants in the absence of adrenoceptors. Pepin and coworkers (1992) have demonstrated increased GR promoter activity in fibroblast cells that are devoid of aminergic receptors, and other studies (Rossby et al. 1995; Eiring and Sulser 1997) found that increased hippocampal GR mRNA expression is independent of increased adrenoceptor stimulation and, in general, independent of norepinephrine bioavailability. In this context, another interesting aspect that has been introduced by Pariante et al. (1997), is that GR trafficking can be induced by antidepressants independently of ligand binding and receptor activation, thus pointing to an interaction of antidepressants with chaperones, and an interference with the energy supply (ATP) necessary to maintain corticosteroid receptors in a hormone-binding mode through energy-requiring activities of chaperones.
In vivo experiments showed that rats, when treated for five weeks with different antidepressants (MAO inhibitors, SSRIs, norepinephrine reuptake inhibitors, serotonin reuptake enhancer), displayed decreased baseline and stress-induced levels of plasma ACTH and corticosterone. Upon analyzing the capacity of MR and GR in the hippocampus of these rats, it was found that the first change was seen in MR binding, which increased after one week of treatment (Reul et al. 1993, 1994, and unpublished results). In the light of the inhibitory effect of MRs on HPA activity which is reflected by studies employing MR antagonists in rats (Spencer et al. 1998) and humans (Dodt et al. 1993; Young et al. 1998) or MR antisense in rats (Reul et al. 1997), the observed upregulation of MR capacity seems to be a first step necessary for the inhibition of hypothalamic CRH neurons. This effect on MR is followed by increased GR capacity (Brady et al. 1991; Seckl and Fink 1992; Reul et al. 1993, 1994).
The physiological significance of MR function is further underscored by studies that showed that acute stress in the hippocampus is associated with increased hippocampal (CA1) MR density and function, an effect that is mediated by CRH and associated with an increased inhibitory tone on HPA activity (Gesing et al., unpublished results). The MR-upregulating effect of antidepressants and the subsequent reduction of HPA overactivity in depressed patients points toward the importance of appropriate MR function. This is further supported by a clinical trial in which antidepressant response to amitriptyline in major depression was impaired by coadministration of spironolactone, an MR antagonist (Hundt et al., unpublished results, cited in Holsboer 1999).
The effects of antidepressants on GR function have been studied using the transgenic mouse that expresses GR antisense (see Section III). After long-term treatment with moclobemide, a reversible inhibitor of MAO A, these mutated mice not only showed normalized HPA activity but also changes in several tests of anxiety and memory (Montkowski et al. 1995). Some of these behavioral alterations may be explained by the recent electrophysiological observation that in these animals the threshold for induction of hippocampal LTP is shifted towards low stimulation frequencies by long-term treatment with moclobemide (Steckler et al. 2000b).
Because long-term administration of moclobemide suppresses HPA activity and activates cAMP-mediated CREB phosphorylation via PKA, the expression of BDNF may be enhanced and followed by a profound alteration in synaptic efficiency (Korte et al. 1995, 1996; Chen et al. 1999; Kafitz et al. 1999). Furthermore, in these transgenic mice, morphine-induced mesolimbic release of serotonin and dopamine as well as the psychomotor-stimulant effects of morphine were enhanced. This is in accordance with the well-established modulation of these neurotransmitters by GR and their modulation of responses to drugs of abuse. After long-term treatment with moclobemide, these neurochemical and behavioral abnormalities disappeared (Sillaber et al. 1998). In addition, tranylcypromin, an irreversible nonselective MAO-inhibitor, was found to induce the AP-1 complex, probably indicating stimulation of a large number of genes by antidepressants (Hope et al. 1994). As outlined above, AP-1 is negatively modulated by GR, whose function is most likely enhanced by this antidepressant drug. These findings support the notion that antidepressants act by improving the negative feedback capacity of the HPA system at various levels and that the setpoint of the HPA system activity is modified in a way that “buffers” the hormonal response to stressors. This may represent one mechanism by which patients who have recovered from a depressive episode may be protected by long-term antidepressant treatment against further stress-triggered relapses.
CRH Receptor Antagonists
If one assumes that normalization of an altered HPA setpoint is an essential mechanism for antidepressant drug action, the question arises as to how this goal is achieved. One explanation involves the well founded β-adrenoceptor desensitization and decrease in cAMP-mediated phosphorylation of transcription factors including CREB, following antidepressant treatment. If this effect of antidepressants, which to date has only been demonstrated in vivo for venlafaxin (Rossby et al. 1999), in vitro, however, for several other antidepressants (Schwaninger et al. 1995), is a general phenomenon of antidepressants, then this decreased activation of CREB would result in decreased CRH expression via CRE (see above). An alternative treatment approach would, therefore, be to either suppress the behavioral symptoms of enhanced CRH by CRH receptor antagonist or to block the many untoward effects excessive corticosteroid secretion may have by administration of GR antagonists.
One recently developed pyrazolopyrimidine, R121919, is a compound with high affinity for CRH-R1 that is now clinically tested and appears to not suppress stress-induced HPA response: patients and controls treated with varying dosages of R121919 did not have an impaired CRH-elicited ACTH and cortisol release (Zobel et al. 2000). This is consistent with the selectivity of such compounds for CRH-R1, leaving pituitary CRH-R2 still responsive to CRH challenges, which in conjunction with other ACTH secretagogues such as vasopressin may override the CRH-R1 blockade at corticotrophs. This is in accord with a report by Sánchez et al. (1999), who found that CRH2-R exist at primate corticotrophs. In addition, the possibility exists that the doses used in human studies still leaves sufficient CRH-R1 available for CRH-elicited ACTH response.
The first open label trial with a CRH1-R antagonist observed significant reductions in depression and anxiety scores using both clinician and patient ratings, suggesting that this type of compound may have considerable therapeutical potential. The question of whether CRH-R1 antagonists resolve the entire depressive syndrome or only several stress-related symptoms, such as pathological anxiety, loss of appetite and sexual drive, sleep disturbance, psychomotor and cardiovascular changes, etc. as found in animal experiments, remains to be validated in controlled clinical studies. Another important aspect are the consequences of CRH-R1 antagonist withdrawal. Long-term CRH-R1 antagonist treatment may result in receptor upregulation and enhanced CRH secretion in a similar way as was seen in CRH-R1 knockout mice where CRH accumulation occurs in all areas in which CRH-R1 is normally expressed, e.g., in the amygdala. After cessation of long-term treatment, patients with depression may have upregulated CRH1 receptors and accumulated ligand (CRH), which together may increase liability for relapse, possibly making tapering of the drug necessary (Zobel et al. 2000).
Cortisol Synthesis Inhibitors
The other alternative focusses on reduction of cortisol either by cortisol synthesis inhibitors or by blocking their action by antagonizing their effects at receptors and has only been investigated in a small number of studies. Administration of metyrapone results in an inhibition of hydroxylation at position C11 of the steroid molecule, thus preventing synthesis of cortisol and corticosterone. Metyrapone treatment has produced antidepressant-like behavioral changes in two frequently used rodent models for screening antidepressive compounds, the forced swim-test and the olfactory-bulbectomized rat (Healy et al. 1999).
Metyrapone and the antidepressant desimipramine produced similar effects in these models, in agreement with a clinical study by O'Dwyer and coworkers (O'Dwyer et al. 1995), who confirmed the antidepressant potential of metyrapone in a placebo-controlled trial already suggested in numerous exploratory studies. This clinical effect seems to contradict the notion that decreased cortisol-mediated feedback may elicit increased CRH in all those brain areas where CRH gene expression is negatively controlled by GR. It was hypothesized that such an increase in CRH may either directly or indirectly precipitate some of the cardinal symptoms of depression. However, as discovered in a series of studies by Patchev et al. (1994, 1996, 1997), the additional bioavailability of non C11-hydroxylated adrenal steroids increases the pool of so-called neuroactive steroids (such as tetrahydro11-deoxy-cortisol or tetrahydro11-deoxycorticosterone) which can exert a number of neuroendocrine and behavioral symptoms such as reduction of anxiety-like behavior and suppression of CRH expression. These results indicate that neuroactive steroids may be able to compensate for the loss of corticosterone (or cortisol in humans) following metyrapone. In this context it is of interest that these neuroactive steroids may exert both genomic and nongenomic effects (Rupprecht et al. 1993) and that the concentration of these steroids is modulated by antidepressants in animals and humans (Romeo et al. 1998; Uzunova et al. 1998; Ströhle et al. 2000).
Other strategies comprise treatments with aminogluthetimide, which is a less selective inhibitor of cortisol biosynthesis, and with ketokonazole. Aminogluthetimide blocks 3β-steroiddehydrogenase and 11β-hydroxylase. Ketokonazole, in addition to blocking adrenal steroid synthesis, inhibits cytochrome P450-dependent enzymes (11-hydroxylase, 17-hydroxylase, C17,20 lyase) similar to aminogluthetimide and has direct inhibitory effects on other anterior pituitary cells than corticotrophs (Stalla et al. 1989) thus, for example, affecting gonadal steroid secretion. Consistent with predictions from open label studies (e.g., Murphy 1991; Thakore and Dinan 1995), a recent double blind placebo-controlled study suggested that ketokonazole is superior to placebo among hypercortisolemic depressed patients (Wolkowitz et al. 1999). Here again, it is difficult to dissociate the effects of ketokonazole from the effects of other adrenal steroids which are produced at much higher rates because of the enzyme blockade. Interestingly, in the study by Wolkowitz and coworkers (1999) pregnenolone sulfate which is a negative allosteric modulator of the GABAA receptor is increased eightfold by ketokonazole. Thus, ketokonazole may exert psychotropic effects through increased pregnenolone which has been reported to improve cognitive impairment in animals (Flood et al. 1995).
Appreciation of studies examining the effects of corticosteroid synthesis inhibitors thus requires recognition of the very complex changes in steroid metabolism which may be induced by the applied drugs; these changes can result in a manifold of different psychotropic effects. Controlled studies evaluating the potential benefits of metyrapone either given alone or in combination with standard antidepressants in depression are warranted.
Corticosteroid Receptor Antagonists
Blockade of corticosteroid receptors as a treatment strategy has been poorly studied due to the lack of specific antagonists. Preliminary results from Murphy et al. (1993) suggested that mifepristone (RU 486) may be a useful treatment approach in major depression. Theoretically, mifepristone may be particularly effective in psychotic depression where the HPA system is most frequently hyperactive and where cortisol induced inhibition of dopamine metabolism may be involved in the pathogenesis of this subtype of depression (Schatzberg et al. 1985). In fact, trimipramin, an effective antidepressant monotherapy of psychotic depression, suppresses hypersecretion of cortisol very effectively (Holsboer-Trachsler et al. 1994; Sonntag et al. 1996).
In contrast to GR antagonism, more substantial data are available for the MR antagonist spironolactone, which if coadministered with the antidepressant amitriptyline impairs antidepressant drug response (Hundt et al., unpublished, cited in Holsboer 1999). The explanation for this finding is related to the studies discussed above, supporting the notion that the first neuroendocrine response to antidepressants is an increase in hippocampal MR, which apparently is required to initiate the cascade that ultimately leads to clinical response (Reul et al. 1993, 1994). Notably, MR agonists enhance hypothalamic CRH release through their hippocampal action. Selective GR antagonists most likely enhance CRH secretion from the hypothalamus which is in line with data from Tronche and coworkers (1999) where a neuron-specific deletion of the GR led to an increase in CRH and PVN of these mice. In other brain areas, e.g., in those where CRH is activated by GR, treatment with GR antagonists may repress CRH gene activity. Clinical trials are therefore needed to clarify the therapeutic potential of GR antagonists in depression and related disorders.
It is not fully clear, however, how disturbed corticosteroid receptor signaling can be influenced therapeutically. Conservatively, administration of a CRH-R1 antagonist is expected to shorten the onset of co-administered antidepressants. Such drugs may also act as antidepressants when administered as monotherapy. Similarly, GR antagonists might not be effective enough as a monotherapy, but they may be a worthwhile adjunct to antidepressants. Whether corticosteroid synthesis inhibitors have therapeutic potential is an open question, since these drugs induce many other psychotropic steroid metabolites. Some studies, however, suggest that metyrapone may taper untoward effects of stress under experimental conditions in rats (Calvo et al. 1998) as well as in patients with cancer (Deguchi et al. 1998).
The examples discussed in this article support the hypothesis that impaired central stress hormone regulation is causally involved in the development and course of depression. In addition, experimental evidence is provided, suggesting that the mechanism of action of antidepressants includes normalization of HPA activity. This hypothesis can now be tested clinically as well as in basic laboratories. While clinical studies are needed to test whether specific intervention at various levels of stress hormone regulation, i.e., CRH-R1 or GR antagonists are equal or superior to current antidepressants, basic studies need to elaborate intracellular signaling and DNA effects of antidepressants and how stress hormones interfere.
The advent of the detailed human genome map in the near future combined with the use of the techniques that allow gene expression profiling on a large scale in a short time offers the possibility to discover novel candidate drug targets. Once identified, the possible relevance of such candidate genes can be verified by generating mouse mutants where the corresponding gene had been inactivated. Only if a relevant phenotype emerges from behavioral screening, a more detailed analysis and the search for molecules that modulate expression of the respective gene or its nuclear effects is warranted.
Techniques that allow large scale analysis of the proteom, and in particular transcription factor activation by phosphorylation in a similar way to that presently used for profiling of gene activation by DNA microarrays still need to be developed. The clinical observations pointing to stress hormone dysregulation causing depression on the one hand and the manifold cellular and nuclear effects of hormone-activated GR and MR on the other, predicts that CR-regulated genes and derived proteins may ultimately be identified as optimal drug targets for depression and possibly other stress-related disorders. Drugs that will emerge from new strategies in biomedicine are likely to be more selectively targeted to specific elements of the HPA system than standard antidepressants. These new drugs promise to act much faster, however only in cases where the central stress hormone system is defunct.
Because these changes in stress-related neuropeptide and corticosteroid secretion are not uniform in the CNS, but subjected to a neuroanatomical microheterogeneity, i.e., may not affect all brain areas in the same way, it seems important to keep in mind that peripheral measures of the pituitary-adrenocortical activity do not necessarily reflect alterations in the release of CR-regulated neuropeptides such as CRH, e.g., in the central amygdala, locus coeruleus, or prefrontal cortex. Therefore, the challenge for clinical research is to develop laboratory tests that allow to specifically identify those patients who may potentially benefit from a therapeutic strategy that specifically targets the central stress hormone system.
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Holsboer, F. The Corticosteroid Receptor Hypothesis of Depression. Neuropsychopharmacol 23, 477–501 (2000). https://doi.org/10.1016/S0893-133X(00)00159-7
- Corticosteriod receptors
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