Abstract
Characterization of a cDNA library, enriched for fragments of genes preferentially expressed in the embryonic day 14.5 mouse forebrain as compared to the adult forebrain, was performed using DNA sequencing and in situ RNA hybridization to sections of mouse embryos. One hundred and thirty different clones have been identified using DNA sequencing. Only 24 of these show homology or identity with either nucleotide or peptide databases. Sixty-five of the clones have also been evaluated by in situ RNA hybridization. Twenty-one clones show either enhanced or regionally specific CNS expression. A nearly full length cDNA was isolated and sequenced for one regionally restricted clone. This novel gene encodes a protein that has strong homology with the Brachyury protein, a known transcriptional regulator that is important in embryogenesis.
SMS has been supported by the NIH Training Grant in Molecular Approaches to Mental Illness and the Veterans Administration Fellowship in Biological Psychiatry.
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Smiga, S., Bulfone, A. & Rubenstein, J. Identification Of Novel Developmentally Regulated Genes By Characterization Of A Subtracted Embryonic Mouse Forebrain Library. Neuropsychopharmacol 11, 285 (1994). https://doi.org/10.1038/sj.npp.1380215
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DOI: https://doi.org/10.1038/sj.npp.1380215