Abstract
ON the findings in some yet unpublished studies on the reducing power of ascorbic acid in phosphate-chloride mixtures, we have developed a specific method for demonstrating ascorbic acid in very weak concentrations. If a little ascorbic acid is added to a solution of methylene blue and the solution is exposed to strong light, the colour will disappear completely within 30 seconds. This decolourisation is promoted by low pH and the presence of sodium chloride. Examination for serum ascorbic acid is therefore made with a stock solution consisting of KH2PO4 9 gm., NaCl 2 gm., c. 0·004 per cent methylene blue solution in 100 ml. 0·1 ml. of this solution is added to 0·9 ml. serum.
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LUND, H., LIECK, H. A Specific Reaction for the Qualitative and Quantitative Determination of Ascorbic Acid in Serum. Nature 137, 784 (1936). https://doi.org/10.1038/137784a0
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DOI: https://doi.org/10.1038/137784a0
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