Abstract
IN a recent issue of NATURE1 Barton-Wright and McBain give results of experiments on the precipitation of virus from infected tobacco juice. The method they used was that of Vinson and Petre, which consists essentially of the precipitation of the protein and other materials from the plant juice with basic lead acetate and the subsequent removal of the virus by elution with potassium-hydrogen phosphate solution. Barton-Wright found that if the mixed phosphate eluate be acidified to a pH of 5 (which means, in effect, the conversion of the alkaline to the acid phosphate, KH2PO4) and 2 volumes of acetone added, a precipitate is thrown down, which is partly colloidal and partly crystalline. The colloidal material is largely protein in nature and is rich in virus. The crystals also contain virus.
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NATURE, 132, 1003, Dec. 30, 1933.
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CALDWELL, J. Possible Chemical Nature of Tobacco Mosaic Virus. Nature 133, 177 (1934). https://doi.org/10.1038/133177b0
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DOI: https://doi.org/10.1038/133177b0
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