Abstract
G protein-activated inwardly rectifying K+ channels (GIRK, also known as Kir3) are activated by various G protein-coupled receptors. GIRK channels play an important role in the inhibitory regulation of neuronal excitability in most brain regions and the heart rate. Modulation of GIRK channel activity may affect many brain functions. Here, we report the inhibitory effects of various antidepressants: imipramine, desipramine, amitriptyline, nortriptyline, clomipramine, maprotiline, and citalopram, on GIRK channels. In Xenopus oocytes injected with mRNAs for GIRK1/GIRK2, GIRK2 or GIRK1/GIRK4 subunits, the various antidepressants tested, except fluvoxamine, zimelidine, and bupropion, reversibly reduced inward currents through the basal GIRK activity at micromolar concentrations. The inhibitions were concentration-dependent with various degrees of potency and effectiveness, but voltage- and time-independent. In contrast, Kir1.1 and Kir2.1 channels in other Kir channel subfamilies were insensitive to all of the drugs. Furthermore, GIRK current responses activated by the cloned A1 adenosine receptor were similarly inhibited by the tricyclic antidepressant desipramine. The inhibitory effects of desipramine were not observed when desipramine was applied intracellularly, and were not affected by extracellular pH, which changed the proportion of the uncharged to protonated desipramine, suggesting its action from the extracellular side. The GIRK currents induced by ethanol were also attenuated in the presence of desipramine. Our results suggest that inhibition of GIRK channels by the tricyclic antidepressants and maprotiline may contribute to some of the therapeutic effects and adverse side effects, especially seizures and atrial arrhythmias in overdose, observed in clinical practice.
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INTRODUCTION
Imipramine and other similar compounds: desipramine, amitriptyline, nortriptyline, and clomipramine, were the first successful antidepressants and have been widely used for the treatment of depression and other psychiatric disorders, such as panic and obsessive–compulsive disorders, bulimia nervosa, and chronic pain disorder (Baldessarini, 2001). Owing to their chemical structures with a three-ring molecular core, they are referred to as the tricyclic antidepressants. Later, a series of newer antidepressants have been developed: tetracyclic antidepressants, for example, maprotiline; atypical antidepressants, for example, bupropion; and selective serotonin reuptake inhibitors (SSRIs) such as fluoxetine, fluvoxamine, zimelidine, and citalopram (Baldessarini, 2001). Inhibition of norepinephrine and/or serotonin (5-hydroxytryptamine (5-HT)) transporters by antidepressants in the brain is generally thought to have important implications in their therapeutic effects (Baldessarini, 2001). In contrast, the interaction of antidepressants with muscarinic, α1 adrenergic, and H1 histamine receptors is involved in some of the adverse side effects (Baldessarini, 2001). It has also been shown that antidepressants inhibit the functions of several other receptors and ion channels, such as 5-HT2C (Ni and Miledi, 1997) and 5-HT3 receptors (Fan, 1994), nicotinic acetylcholine receptors (García-Colunga et al, 1997; Maggi et al, 1998), N-methyl-D-aspartate (NMDA) receptor channels (Sernagor et al., 1989), P2X2 receptors (Nakazawa et al, 1999), voltage-gated Ca2+, Na+, and K+ channels (Ogata et al, 1989; Mathie et al, 1998; Pancrazio et al, 1998; Teschemacher et al, 1999; Yeung et al, 1999; Deák et al, 2000; Choi et al, 2001; Cuellar-Quintero et al, 2001), Ca2+-activated K+ channels (Kamatchi and Ticku, 1991; Lee et al, 1997; Dreixler et al, 2000; Terstappen et al, 2001), and Cl− channels (Maertens et al, 1999, 2002). The effects might also be involved in the molecular and cellular mechanisms underlying some of the therapeutic effects and side effects of various antidepressants.
G protein-activated inwardly rectifying K+ (GIRK) channels (also known as Kir3 channels) are members of a family of inward-rectifier K+ (Kir) channels that include seven subfamilies (Doupnik et al, 1995; Reimann and Ashcroft, 1999). Four GIRK channel subunits have been identified in mammals (Kubo et al, 1993b; Lesage et al, 1995; Wickman et al, 1997). Neuronal GIRK channels are predominantly heteromultimers composed of GIRK1 and GIRK2 subunits in most brain regions (Kobayashi et al, 1995; Lesage et al, 1995; Karschin et al, 1996; Liao et al, 1996) or homomultimers composed of GIRK2 subunits in the substantia nigra (Inanobe et al, 1999), whereas atrial GIRK channels are heteromultimers composed of GIRK1 and GIRK4 subunits (Krapivinsky et al, 1995). Various G protein-coupled receptors, such as M2 muscarinic, α2 adrenergic, D2 dopaminergic, 5-HT1A, opioid, nociceptin/orphanin FQ and A1 adenosine receptors, activate GIRK channels (North, 1989; Ikeda et al, 1995, 1996, 1997) through direct action of G protein βγ subunits (Reuveny et al, 1994). In addition, ethanol activates GIRK channels independent of G protein-coupled signaling pathways (Kobayashi et al, 1999; Lewohl et al, 1999). Activation of GIRK channels causes membrane hyperpolarization, and thus the channels play an important role in the inhibitory regulation of neuronal excitability and heart rate (North, 1989; Signorini et al, 1997; Wickman et al, 1998). Therefore, modulators of GIRK channel activity may affect many brain and cardiac functions. Using the Xenopus oocyte expression system, we demonstrated that various antipsychotic drugs including thioridazine and clozapine and an SSRI antidepressant drug, fluoxetine, inhibited GIRK channels (Kobayashi et al, 1998, 2000, 2003). Imipramine, a tricyclic antidepressant of the dibenzazepines, is chemically similar to the antipsychotic drugs thioridazine, a phenothiazine, and clozapine, a dibenzodiazepine. Therefore, we hypothesized that tricyclic antidepressants might interact with GIRK channels. In the present study, we examined the effects of various antidepressants including tricyclic antidepressants on brain-type GIRK1/2 and GIRK2 channels and cardiac-type GIRK1/4 channels by using the Xenopus oocyte expression assay.
MATERIALS AND METHODS
Preparation of Specific mRNAs
Plasmids containing the entire coding sequences for the mouse GIRK1, GIRK2, and GIRK4 channel subunits, and the Xenopus A1 adenosine (XA1) receptor, were obtained by using the polymerase chain reaction method as described previously (Kobayashi et al, 1995, 2000, 2002). In addition, cDNAs for rat Kir1.1 in pSPORT and mouse Kir2.1 in pcDNA1 were provided by Dr Steven C Hebert and Dr Lily Y Jan, respectively. These plasmids were linearized by digestion with an appropriate enzyme as described previously (Ho et al, 1993; Kubo et al, 1993a; Kobayashi et al, 2000), and the specific mRNAs were synthesized in vitro by using the mMESSAGE mMACHINETM In vitro Transcription Kit (Ambion, Austin, TX, USA).
Electrophysiological Analyses
Adult female Xenopus laevis frogs were purchased from Copacetic (Soma, Aomori, Japan) and maintained in the laboratory until used. Frogs were anesthetized by immersion in water containing 0.15% tricaine (Sigma Chemical Co., St Louis, MO, USA). A small incision was made on the abdomen to remove several ovarian lobes from the frogs that were humanely killed after the final collection. Oocytes (Stages V and VI) were isolated manually from the ovary and maintained in Barth's solution (Kobayashi et al, 2002). Xenopus laevis oocytes were injected with mRNA(s) for GIRK1/GIRK2 or GIRK1/GIRK4 combinations (each ∼0.4 ng), GIRK2 (∼5 ng), Kir1.1 (∼5 ng) or Kir2.1 (∼0.5 ng) and/or XA1 (∼10 ng). The oocytes were incubated at 19°C in Barth's solution, and defolliculated following treatment with 0.8 mg ml−1 collagenase as described previously (Kobayashi et al, 2002). Whole-cell currents of the oocytes were recorded from 2 to 10 days after the injection with a conventional two-electrode voltage clamp (Kobayashi et al, 1999; Ikeda et al, 2003). The membrane potential was held at −70 mV, unless otherwise specified. Microelectrodes were filled with 3 M KCl. The oocytes were placed in a 0.05 ml narrow chamber and superfused continuously with a high-potassium (hK) solution (composition in mM: KCl 96, NaCl 2, MgCl2 1, CaCl2 1.5, and HEPES 5, pH 7.4 with KOH) or a K+-free high-sodium (ND98) solution (composition in mM: NaCl 98, MgCl2 1, CaCl2 1.5, and HEPES 5, pH 7.4 with NaOH) at a flow rate of 2.5 ml min−1. For examining the effect of intracellular desipramine, 23 nl of 30 mM desipramine or 30 mM lidocaine N-ethyl bromide (QX-314) dissolved in distilled water was injected into an oocyte by using a Nanoliter injector (World Precision Instruments, Sarasota, FL, USA) as described previously (Kobayashi et al, 2003), and the oocyte currents were continuously recorded for approximately 30−40 min. Owing to a volume of ∼1 μl in the oocyte, the intracellular concentration of desipramine was presumed as ∼674 μM. In the hK solution, the K+ equilibrium potential (EK) was close to 0 mV, and inward K+ current flow through Kir channels was observed at negative holding potentials. Data were fitted to a standard logistic equation by using KaleidaGraph (Synergy Software, Reading, PA, USA) for analysis of concentration–response relationships. The EC50 value, which is the concentration of a drug that produces 50% of the maximal current response for that drug, the IC25 and IC50 values, which are the concentrations of a drug that reduces control current responses by 25 and 50%, respectively, and the Hill coefficient (nH) were obtained from the concentration–response relationships.
Statistical Analysis of Results
The values obtained are expressed as the mean±SEM, and n is the number of oocytes tested. Statistical analysis of differences between groups was carried out by using paired t-test, Student's t-test, one-way ANOVA or two-way factorial ANOVA, followed by Bonferroni/Dunn post hoc test. A probability of 0.05 was taken as the level of statistical significance.
Compounds
All the antidepressants tested, except fluvoxamine (Tocris Cookson, Bristol, UK), and adenosine were purchased from Research Biochemicals Inc (Natick, MA, USA). Maprotiline, fluvoxamine, and citalopram were dissolved in dimethyl sulfoxide (DMSO). Other drugs were dissolved in distilled water. The stock solutions of all of the compounds were stored at −30°C until used. Ethanol was purchased from Wako Pure Chemical Industries (Osaka, Japan). Each compound was added to the perfusion solution in appropriate amounts immediately before the experiments.
RESULTS
Inhibition of GIRK Channels by Antidepressants
To investigate whether various antidepressants interact with brain-type GIRK1/2 and cardiac-type GIRK1/4 channels, we conducted Xenopus oocyte expression assays. In oocytes co-injected with GIRK1 and GIRK2 mRNAs, basal GIRK currents, which are known to depend on free G protein βγ subunits present in the oocytes because of the inherent activity of G proteins (Dascal, 1997), were observed under the conditions of a hK solution containing 96 mM K+ and negative membrane potentials (Kobayashi et al, 2003; Figure 1a). Application of 300 μM imipramine, desipramine, amitriptyline or nortriptyline, tricyclic antidepressants, immediately and reversibly caused a reduction of the inward currents through the expressed GIRK channels in the hK solution (Figure 1a). The current responses were abolished in the presence of 3 mM Ba2+, which blocks the Kir channel family including GIRK channels (n=3; data not shown). None of the antidepressants produced a significant response in the K+-free ND98 solution containing 98 mM Na+ instead of the hK solution (n=3; data not shown), suggesting that the antidepressant-sensitive current components show K+ selectivity. In uninjected oocytes, the antidepressants tested, even at the highest concentrations used, and 3 mM Ba2+ caused no significant response (Figure 1c; n=4 and 10, respectively), suggesting no effect of the antidepressants and Ba2+ on intrinsic oocyte channels. These results suggest that the tricyclic antidepressants inhibit GIRK1/2 channels. In addition, since the magnitudes of inhibition of basal GIRK currents by 3 mM Ba2+ were almost equal to those by 5 mM Ba2+ in oocytes expressing GIRK channels, the 3 mM Ba2+-sensitive current components (1048.6±60.2 nA, n=66) were considered to correspond to the magnitudes of GIRK1/2 currents. Maprotiline, which is a tetracyclic antidepressant related structurally to tricyclic antidepressants, also inhibited basal GIRK currents by 44.8±4.9% at 100 μM (n=8), whereas citalopram, an SSRI, and bupropion had a small or no effect on the currents (21.7±2.9% inhibition and 4.7±0.6% inhibition of the 3 mM Ba2+-sensitive current component at 100 μM, n=6 and 3, respectively). In addition, application of DMSO, the solvent vehicle, at the highest concentration (0.3%) used had no significant effect on the current responses in oocytes co-injected with GIRK1 and GIRK2 mRNAs (n=4; data not shown). Similarly, in oocytes co-injected with GIRK1 and GIRK4 mRNAs (Figures 1b), basal GIRK currents were observed under the same conditions, and the current components sensitive to 3 mM Ba2+ were 880.2±71.3 nA (n=64). The tricyclic antidepressants tested and maprotiline inhibited basal GIRK1/4 currents (Figures 1b, 2b), suggesting that the antidepressants also inhibit GIRK1/4 channels. However, citalopram and bupropion had little or no effect on basal GIRK1/4 currents (6.5±1.7% inhibition and 3.7±3.2% inhibition of the 3 mM Ba2+-sensitive current component at 100 μM, n=3 and 8, respectively).
We next investigated the concentration–response relationship of the inhibitory effects of the various antidepressants on GIRK channels expressed in Xenopus oocytes, compared with the current components sensitive to 3 mM Ba2+, which fully blocked basal GIRK currents (Kobayashi et al, 2002). Figure 2 shows that inhibition of GIRK1/2 and GIRK1/4 channels by the antidepressants is concentration-dependent with distinctive potency and effectiveness at micromolar concentrations. All the antidepressants inhibited both types of GIRK channels to a limited extent even at high concentrations. Table 1 shows the EC50 and nH values obtained from the concentration–response relationships for the antidepressants tested and the percentage inhibition of the GIRK currents by the drugs at the highest concentrations tested. The rank order of the inhibition of GIRK1/2 and GIRK1/4 channels by 100 μM of these drugs was as follows: desipramine > clomipramine ⩾ imipramine > nortriptyline = amitriptyline ⩾ maprotiline ≫ citalopram ≫ bupropion for GIRK1/2 channels and desipramine > clomipramine ⩾ amitriptyline > maprotiline ⩾ nortriptyline > imipramine ≫ citalopram = bupropion for GIRK1/4 channels. To further compare the effects of the antidepressants on GIRK channels, we also calculated the drug concentrations required to inhibit the GIRK currents by 25 or 50% (Table 1). Among these antidepressants, desipramine, a tricyclic antidepressant, was the most effective inhibitor of GIRK1/2 and GIRK1/4 channels. The effects of imipramine and clomipramine on GIRK1/2 channels were similar, and those of amitriptyline, nortriptyline, and maprotiline were similar on GIRK1/2 channels and GIRK1/4 channels. The effects of desipramine, amitriptyline, and maprotiline on GIRK1/2 channels were similar to those on GIRK1/4 channels, respectively, although inhibition of GIRK1/2 channels by maprotiline at high concentrations was more effective than that of GIRK1/4 channels. In addition, inhibition of GIRK1/2 channels by imipramine, nortriptyline, clomipramine, and citalopram was more effective than that of GIRK1/4 channels, respectively (Figure 2 and Table 1).
Although GIRK channels in the brain are predominantly GIRK1/2 heteromultimers (Liao et al, 1996), GIRK channels in the substantia nigra and ventral tegmental area are GIRK2 homomultimers (Karschin et al, 1996; Inanobe et al, 1999). To further address the functional relationship between various antidepressants and neuronal GIRK channels, we investigated the effects of the antidepressants on the homomeric channels (Figure 3). The inhibitory effects of the tricyclic antidepressants used and maprotiline on GIRK2 channels in oocytes expressing GIRK2 channels were less effective than those on GIRK1/2 channels (p<0.01, significant interaction between the channel effect and the effect of the antidepressants, two-way factorial ANOVA; and p<0.01, significant differences between the effects of each antidepressant on GIRK1/2 and GIRK2 channels, Bonferroni/Dunn post hoc test). For SSRIs, the inhibitory effect of citalopram on GIRK2 channels was similar to that on GIRK1/2 channels (23.9±6.9% inhibition of the 3 mM Ba2+-sensitive current component at 100 μM, n=4), whereas fluvoxamine and zimelidine had little effect on GIRK2 channels (5.3±2.7% inhibition and 2.9±0.3% inhibition of the 3 mM Ba2+-sensitive current component at 100 μM, n=3 and 3, respectively), as were the cases for GIRK heteromeric channels (Kobayashi et al, 2003). In addition, bupropion had little effect on GIRK2 channels (3.9±1.8% inhibition of the 3 mM Ba2+-sensitive current component at 100 μM, n=4).
Furthermore, we examined whether the various antidepressants could interact with Kir1.1, an ATP-regulated inwardly rectifying K+ channel, and Kir2.1, a constitutively active inward rectifier K+ channel, in other Kir channel subfamilies (Figure 3). In oocytes expressing Kir1.1 or Kir2.1 channels, application of each antidepressant at 100 μM had no significant effect on the inward currents through the channels in the hK solution (n⩾3 for Kir1.1 and n=3 for Kir2.1).
Characteristics of Antidepressant Inhibition of GIRK Channels
As the various tricyclic antidepressants tested acted as inhibitors at GIRK channels in the present study, we further investigated the effect of the tricyclic antidepressants, particularly desipramine, in more detail. The instantaneous GIRK1/2 currents elicited by the voltage step to −100 mV from a holding potential of 0 mV diminished in the presence of 100 μM desipramine (Figure 4a). The percentage inhibition of the steady-state GIRK current at the end of the voltage step by desipramine was not significantly different from that of the instantaneous current (paired t-test, p>0.05; n=4 at −80, −100, and −120 mV, respectively). These results suggest that the channels were inhibited by desipramine primarily at the holding potential of 0 mV in a time-independent manner. Furthermore, similar results were obtained for imipramine, amitriptyline, nortriptyline, and clomipramine (data not shown).
Like 3 mM Ba2+-sensitive currents corresponding to basal GIRK currents, desipramine-sensitive currents in oocytes expressing GIRK channels increased with negative membrane potentials, and the current–voltage relationships showed strong inward rectification (Figure 4b), indicating a characteristic of GIRK currents. Furthermore, similar results were obtained for imipramine, amitriptyline, nortriptyline, clomipramine, and maprotiline (data not shown).
The percentage inhibition of GIRK currents by 100 μM desipramine was measured at membrane potentials between −100 and −20 mV. For GIRK1/2 and GIRK1/4 channels, the percentage inhibition showed no significant difference across the voltages (p>0.05, one-way ANOVA; Figure 4c). These results suggest that inhibition of GIRK channels by desipramine was voltage-independent. Furthermore, similar results were obtained for imipramine, amitriptyline, nortriptyline, clomipramine, and maprotiline (data not shown).
At physiological pH or below, desipramine exists mainly in a protonated form, and the proportion of the uncharged form increases with an increase in pH, because of a pKa value of 10.2 (Green, 1967). We examined whether changes in pH affect the inhibition by desipramine of GIRK channels expressed in oocytes prepared from the same donor. For GIRK1/2 channels, no effect of pH on the inhibition was observed in the concentration–response relationships for desipramine (p>0.05 at each concentration, n=5, Student's t-test, Figure 5). For GIRK1/4 channels, similar results were obtained at 30 and 100 μM desipramine (p>0.05, n=4, Student's t-test). These results suggest that the inhibition may be mediated by both forms of desipramine with almost the same effectiveness. It also appears unlikely that the inhibition by desipramine is caused by hydrophobic interactions with GIRK channels within the membrane bilayer.
Moreover, we examined the effects of desipramine on GIRK channels activated by G protein-coupled receptors. In oocytes co-expressing GIRK1/2 channels and XA1 receptors (Kobayashi et al, 2002), application of 10 nM adenosine induced inward GIRK currents (Figure 6a). The effects of desipramine were evaluated by measuring the amplitude of the adenosine-induced current response during application of desipramine at different concentrations. The current responses to 10 nM adenosine were reversibly inhibited by desipramine with an IC50 value of 38.6±8.7 μM and an nH value of 0.76±0.06 (n=6, Figure 6a, b). The percentage inhibition by desipramine was similar to that of basally active GIRK1/2 channels (p>0.1 at each concentration, Student t-test), suggesting the interaction of desipramine with GIRK channels. In addition, the adenosine-induced GIRK currents were not significantly affected by intracellularly applied desipramine (90.8±10.6% of pretreated control current, paired t-test, p>0.1, n=5, Figure 6c), whereas the GIRK currents were significantly inhibited by intracellularly applied QX-314 as reported previously (Zhou et al, 2001; Kobayashi et al, 2003). The results, therefore, suggest that extracellular desipramine can directly inhibit GIRK channels activated by the XA1 receptors.
GIRK channels are also activated by ethanol independent of G protein signaling pathways (Kobayashi et al, 1999). We next examined the effect of desipramine on ethanol-induced GIRK currents. In oocytes expressing GIRK1/2 channels, the GIRK currents induced by ethanol were attenuated in the presence of desipramine, with an IC50 value of 34.6±13.4 μM and an nH value of 1.10±0.08, in a reversible manner (62.3±4.9% inhibition at 100 μM, n=5; Figure 7). The results, therefore, suggest that desipramine can inhibit GIRK channels activated by ethanol.
DISCUSSION
We demonstrated that all of the tricyclic antidepressants tested, namely, desipramine, imipramine, amitriptyline, nortriptyline, and clomipramine, inhibited brain-type GIRK1/2 and GIRK2 channels and cardiac-type GIRK1/4 channels at micromolar concentrations in a distinctive manner. Furthermore, maprotiline, which also possesses a similar tricyclic structure, inhibited GIRK channels. These results indicate that tricyclic moiety of these antidepressants may be involved in GIRK channel inhibition. Among SSRIs, fluoxetine significantly inhibited GIRK channels even at low micromolar levels, whereas fluvoxamine and zimelidine had little effect on the channels (Kobayashi et al, 2003). In the present study, citalopram weakly inhibited brain-type GIRK1/2 and GIRK2 channels, but not cardiac-type GIRK1/4 channels. These distinctive effects on GIRK channels may be due to the diverse chemical structures of SSRIs. In addition, both types of GIRK channels were insensitive to bupropion, an atypical antidepressant, suggesting that the drugs with the chemical structure similar to bupropion may have no significant effect on GIRK channels.
The inhibition of GIRK channels by the tricyclic and tetracyclic antidepressants tested was concentration-dependent but voltage- and time-independent. The GIRK currents were not completely blocked by the antidepressants even at high concentrations. Our results also suggest that desipramine, a tricyclic antidepressant, acts at the channels from the extracellular side of the cell membrane. On the other hand, blockade by extracellular Ba2+ and Cs+, typical of Kir channel blockers that occlude the pore of the open channel, shows a concentration dependence, a strong voltage dependence and a time dependence with a comparatively small effect on the instantaneous current, but a marked inhibition on the steady-state current at the end of voltage pulses (Lesage et al, 1995). These observations suggest that the antidepressants may probably cause a conformational change in the channels, but not act as open channel blockers of Kir channels like Ba2+ and Cs+. The difference in action mechanism between the antidepressants and Ba2+ may be involved in the incomplete blockade of GIRK channels by the antidepressants. In addition, the inhibitory effects of the tricyclic and tetracyclic antidepressants tested on GIRK2 homomeric channels were weaker than those on GIRK1/2 heteromeric channels. Moreover, Kir1.1 and Kir2.1 channels among members of the Kir channel family were insensitive to all of the antidepressants. Further studies using GIRK/Kir1.1 and GIRK/Kir2.1 chimeric channels and mutant GIRK channels may clarify the critical sites mediating the effects of the antidepressants on GIRK channels.
The therapeutic plasma concentrations of various antidepressants range approximately from 0.7 to 1.1 μM for imipramine, 0.47 to 1.1 μM for desipramine, 0.36 to 0.9 μM for amitriptyline, 0.23 to 0.57 μM for nortriptyline, 0.48 to 1.6 μM for clomipramine, 0.7 to 1.4 μM for maprotiline, and 0.23 to 0.46 μM for citalopram (Baldessarini, 2001). The plasma concentrations after their overdoses become significantly higher than those in the therapeutic use (Prouty and Anderson, 1990; Barbey and Roose, 1998; Rosenstein et al, 1993). As most antidepressants have highly lipophilic properties, they accumulate in the brain (Prouty and Anderson, 1990): namely, the brain-to-plasma ratios are approximately 13−20:1 for imipramine (Besret et al, 1996), 15−25 : 1 for desipramine (Baumann et al, 1983), 10−35 : 1 for amitriptyline (Glotzbach and Preskorn, 1982; Baumann et al, 1984; Miyake et al, 1990), 8−15 : 1 for nortriptyline (Baumann et al, 1984; Miyake et al, 1990), 10−48 : 1 for clomipramine (Friedman and Cooper, 1983; Eschalier et al, 1988), 44−59 : 1 for maprotiline (Miyake et al, 1991), and 4−8 : 1 for citalopram (Kugelberg et al, 2001). In addition, the mean brain concentrations of the tricyclic antidepressants in postmortem humans were approximately 20 times higher than the corresponding blood levels (Prouty and Anderson, 1990). Therefore, the present findings suggest that GIRK channels in the brain may be inhibited by the various tricyclic antidepressants and maprotiline at clinically relevant brain concentrations.
Interestingly, GIRK2-deficient mice showed reduced anxiety and an increase in motor activity (Blednov et al, 2001). Although it is generally thought that the therapeutic effects of antidepressants are primarily due to inhibition of the reuptake of norepinephrine and/or serotonin in the brain, inhibition of neuronal GIRK channels by the antidepressants may contribute to additive therapeutic effects for depression and some psychiatric disorders. Also, antidepressant drugs have various side effects, including sedation, tremor, seizures, orthostatic hypotension, tachycardia, and weight gain (Baldessarini, 2001). Among these side effects, the incidence of seizures is a serious side effect. The risk of seizures for most antidepressants increases with the dose (Rosenstein et al, 1993; Barbey and Roose, 1998; Baldessarini, 2001). The mean plasma concentrations of tricyclic antidepressants in several patients who experienced seizures following overdoses were reported to be approximately eight times higher than those in the therapeutic doses (Boehnert and Lovejoy, 1985; Rosenstein et al, 1993). Therefore, the tricyclic antidepressants and maprotiline at the brain levels after the overdoses may potently inhibit neuronal GIRK channels. As opposed to these drugs, citalopram, which only slightly inhibits GIRK channels even at toxic concentrations, has a lower seizure risk (Barbey and Roose, 1998). The inhibition of GIRK channels leads to depolarize the membrane potential, resulting in an increase in neuronal excitability (Kuzhikandathil and Oxford, 2002). In addition, GIRK2-deficient mice show spontaneous seizures (Signorini et al, 1997). Therefore, potent inhibition of neuronal GIRK channels by some antidepressants may contribute to the cause of the neuropsychiatric toxicity, particularly seizures.
Antidepressant drugs including tricyclic antidepressants and SSRIs show analgesic activity (Messing et al, 1975; Lin et al, 1980; Schreiber et al, 1996; Korzeniewska-Rybicka and Plaznik, 2000; Galeotti et al, 2001). Studies using GIRK2-deficient mice and weaver mutant mice, which have mutant GIRK2 channels insensitive to G proteins and ethanol, have shown that the analgesic effects induced by opioids or ethanol are remarkably reduced in these mice, suggesting that the GIRK channel activation induces analgesia (Kobayashi et al, 1999; Ikeda et al, 2000, 2002; Blednov et al, 2003; Mitrovic et al, 2003). However, our studies have demonstrated that various tricyclic antidepressants and fluoxetine (Kobayashi et al, 2003) inhibit GIRK channels. Therefore, the analgesic effects of these antidepressants may not be mediated by GIRK channels. On the other hand, it has been shown that the analgesic effects of various antidepressant drugs may be caused by interaction with other several targets: voltage-gated Na+ channels (Pancrazio et al, 1998) and voltage-gated, ATP-sensitive and Ca2+-activated K+ channels (Galeotti et al, 2001), by the action of 5-HT (Messing et al, 1975; Lin et al, 1980; Tura and Tura, 1990) and by opioid mechanisms (Reichenberg et al, 1985).
In the heart, acetylcholine released from the stimulated vagus nerve opens atrial GIRK channels via activation of the M2 muscarinic acetylcholine receptor, and ultimately causes slowing of the heart rate (Brown and Birnbaumer, 1990). Sinus tachycardia during tricyclic antidepressant treatment is frequently observed (Baldessarini, 2001). Various tricyclic antidepressants exhibit nanomolar affinity for the receptor (Stanton et al, 1993). The present study demonstrated that micromolar concentrations of various tricyclic antidepressants and maprotiline inhibited cardiac-type GIRK1/4 channels. The heart concentrations of the tricyclic antidepressants are approximately 20–200 times higher than the corresponding blood levels (Elonen et al, 1975; Jandhyala et al, 1977). No exact heart-to-plasma ratio for maprotiline has been reported. However, because maprotiline has a similar tricyclic structure, it may also highly accumulate in the heart. Therefore, atrial GIRK channels may also be inhibited by the antidepressants in clinical practice. Taken together, sinus tachycardia during the treatment with antidepressants may be related to not only antagonism of the M2 muscarinic acetylcholine receptor, but also inhibition of atrial GIRK channels. Moreover, toxic plasma levels after overdoses of tricyclic antidepressants are associated with cardiac side effects including atrial premature contractions and supraventricular tachycardia (Frommer et al, 1987). Cardiac-type GIRK1/4 channels are abundantly present in the atrium of the heart (Krapivinsky et al, 1995). Therefore, greater inhibition of atrial GIRK channels by the antidepressants after overdoses may contribute to the incidence of these atrial arrhythmias in cardiac toxicity. In contrast, the cardiac side-effects of bupropion and citalopram in clinical practice are uncommon (Baldessarini, 2001). These drugs have weak antimuscarinic properties (Baldessarini, 2001) and no significant effect on GIRK1/4 channels (Figure 2b). Therefore, the minimal cardiotoxic effects of bupropion and citalopram may be partly attributable to their lack of any significant effect on atrial GIRK channels, together with their weak antimuscarinic effects.
Desipramine, a norepinephrine reuptake inhibitor, reduces ethanol consumption like SSRIs including fluoxetine (Murphy et al, 1985; Naranjo and Knoke, 2001), suggesting a relation between the antidepressants and ethanol effects. Recently, we demonstrated that fluoxetine inhibited ethanol-induced GIRK1/2 currents (Kobayashi et al, 2003). The present study also demonstrates that desipramine at micromolar concentrations can inhibit GIRK1/2 currents induced by ethanol. However, GIRK2-deficient mice displayed no significant change in ethanol consumption (Blednov et al, 2001). Further studies using mice treated with antisense oligodeoxynucleotides for the GIRK mRNAs, mice lacking other GIRK subunit(s) in addition to the GIRK2 subunit and mutant mice with GIRK channels insensitive to ethanol alone may help to advance the understanding of the antagonism by desipramine of ethanol-induced GIRK effects in vivo as well as the understanding of the functions of GIRK channels.
Lastly, our electrophysiological studies have clarified the functional actions of various psychotropic drugs on GIRK channels using Xenopus oocyte expression assays (Kobayashi et al, 1999, 2000, 2003). Recent studies using GIRK2-deficient mice suggested that GIRK activators may be useful for the treatment of epilepsy and pain (Signorini et al, 1997; Ikeda et al, 2002; Blednov et al, 2003). In addition, since GIRK knockout mice show decreased cocaine self-administration (Morgan et al, 2003), selective GIRK channel inhibitors might be potential agents for the treatment of abusers of cocaine. Therefore, as GIRK channels are considered candidates for clinically relevant targets, it may be important to clarify the pharmacological and physiological effects of various agents interacting with GIRK channels.
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Acknowledgements
We thank Dr Kansaku Baba for cooperation, and Tomio Ichikawa, Kazuo Kobayashi, and Kazuyo Sekikawa for their assistance. We also thank Dr Steven C Hebert for providing the Kir1.1 cDNA, and Dr Lily Y Jan for providing the Kir2.1 cDNA. This work was supported by research grants from the Ministry of Education, Science, Sports and Culture of Japan, the Ministry of Health, Labour and Welfare of Japan, and the RIKEN Brain Science Institute.
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Kobayashi, T., Washiyama, K. & Ikeda, K. Inhibition of G Protein-Activated Inwardly Rectifying K+ Channels by Various Antidepressant Drugs. Neuropsychopharmacol 29, 1841–1851 (2004). https://doi.org/10.1038/sj.npp.1300484
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DOI: https://doi.org/10.1038/sj.npp.1300484
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