Abstract
The BRCA2 gene is involved in recombinational DNA repair and cytokinesis. BRCA2 defects are associated with chromosomal abnormalities, which is a hallmark of genomic instability that contributes to tumorigenesis. Here, we show that downregulation of a BRCA2 interacting protein (BCCIP) in HT1080 cells leads to chromosomal polyploidization, centrosome amplification and abnormal mitotic spindle formation. The BCCIP knockdown cells can enter mitosis and retain spindle checkpoint, but fail to complete cytokinesis. Our data suggest an essential role of BCCIP in the maintenance of genomic integrity.
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Acknowledgements
This research was supported by National Institute of Health grants CA115488 and ES08353 and by the US Army Medical Research and Materiel Command grants DAMD17-02-1-0515 and DAMD17-03-1-0317. We thank the technical support from the flow cytometry and the fluorescence microscopy facility of UNM Cancer Research and Treatment Center.
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Supplementary Information accompanies the paper on the Oncogene Web site (http://www.nature.com/onc).
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Meng, X., Fan, J. & Shen, Z. Roles of BCCIP in chromosome stability and cytokinesis. Oncogene 26, 6253–6260 (2007). https://doi.org/10.1038/sj.onc.1210460
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DOI: https://doi.org/10.1038/sj.onc.1210460
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